Study on Rapid Propagation of Musella lasiocarpa

Key words: 
• Musella lasiocarpa /
• sucker /
• rapid propagation /

Abstract: The suckers were used as explants to perform the rapid propagation of Musella lasiocarpa.The ideal medium prescription for some key procedures,such as the establishment of sterile materials,the proliferation of clustered buds and the rooting,were developed.According to the routine methods of plant tissue culture,the experiments were operated and SAS software was used to analysis the data.The results showed that after the suckers were longitudinally cut and cultured on the medium（MS+2.5 mg·L^-1 6-BA + 30 g·L^-1 sugar） for 20 days,the young adventitious buds continuously appeared;the best proliferation effects could be obtained by transplanting the young buds onto the medium（MS+2.0 mg·L^-1 6-BA + 30 g·L^-1 sugar +10% CW） for multiplication culture,on which the young shoots with deep green color grow normally,and with a proliferation coefficient of 5.47;when the concentration of 6-BA was higher than 2.5 mg·L^-1,the young shoots showed a dehydrated-like growth phase,and the malformed and vitrified shoots increased;the optimum rooting effects could be obtained by transplanting the young shoot onto the medium（1/2MS+1.5 mg·L^-1 NAA +2.5 mg·L^-1 active carbon）,on which the average root number of single plant was 3.07,the average root length was 2.43 cm,and the average rooting percentage was 96.7%.Additionally,when the vessel plantlets of Musella lasiocarpa were transplanted in the mixed substrate（river sand∶surface soil = 1∶1）,the survival percentage of the young plantlets amounted to 93.7% and it grew very well.