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Volume 2 Issue 1
Mar.  2011
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Article Navigation >  Journal of Tropical Biology  > 2011  >  2(1): 42-45

LI Kai, CHEN Xiongting, HONG Lei, GAN Lu. Improving on Mature-type Citrus grandis Stem Cultured in vitro[J]. Journal of Tropical Biology, 2011, 2(1): 42-45. doi: 10.15886/j.cnki.rdswxb.2011.01.010
Citation: LI Kai, CHEN Xiongting, HONG Lei, GAN Lu. Improving on Mature-type Citrus grandis Stem Cultured in vitro[J]. Journal of Tropical Biology, 2011, 2(1): 42-45. doi: 10.15886/j.cnki.rdswxb.2011.01.010
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Improving on Mature-type Citrus grandis Stem Cultured in vitro

doi: 10.15886/j.cnki.rdswxb.2011.01.010

  • 1. Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China;

  • 2. College of Agriculture, Hainan University, Haikou 570228, China

  • Received Date: 2011-01-05
  • The effects of different concentrations of macro-element and combination of plant growth regulator on the mature-type Citrus grandis stem cultured in vitro were compared.The results showed when 3/4MS mediums,in which the 6-BA concentrations were 0.6 mg·L-1-0.8 mg·L-1 and NAA concentrations were 0.8mg·L-1-1.2 mg·L-1,were used,high proliferation rate were obtained;when the concentrations of 6-BA,NAA and GA3 were 0.3 mg·L-1,0.6 mg·L-1 and 0.3 mg·L-1,respectively,multiple shoots’ long-time subculture were feasible.
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Improving on Mature-type Citrus grandis Stem Cultured in vitro

doi: 10.15886/j.cnki.rdswxb.2011.01.010
  • 1. Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China;
  • 2. College of Agriculture, Hainan University, Haikou 570228, China
  • Received Date: 2011-01-05

Abstract: The effects of different concentrations of macro-element and combination of plant growth regulator on the mature-type Citrus grandis stem cultured in vitro were compared.The results showed when 3/4MS mediums,in which the 6-BA concentrations were 0.6 mg·L-1-0.8 mg·L-1 and NAA concentrations were 0.8mg·L-1-1.2 mg·L-1,were used,high proliferation rate were obtained;when the concentrations of 6-BA,NAA and GA3 were 0.3 mg·L-1,0.6 mg·L-1 and 0.3 mg·L-1,respectively,multiple shoots’ long-time subculture were feasible.

LI Kai, CHEN Xiongting, HONG Lei, GAN Lu. Improving on Mature-type Citrus grandis Stem Cultured in vitro[J]. Journal of Tropical Biology, 2011, 2(1): 42-45. doi: 10.15886/j.cnki.rdswxb.2011.01.010
Citation: LI Kai, CHEN Xiongting, HONG Lei, GAN Lu. Improving on Mature-type Citrus grandis Stem Cultured in vitro[J]. Journal of Tropical Biology, 2011, 2(1): 42-45. doi: 10.15886/j.cnki.rdswxb.2011.01.010
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