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Volume 2 Issue 1
Mar.  2011
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Article Navigation >  Journal of Tropical Biology  > 2011  >  2(1): 6-13,20

ZHAI Jinling, XU Yuanfeng, MA Hongwei, CHEN Xufeng, HUANG Xi. Cloning and Sequence Analysis of Gene Encoding Vacuolar-type Na+/H+ Antiporter of Rhizophora stylosa（RsNHX1）[J]. Journal of Tropical Biology, 2011, 2(1): 6-13,20. doi: 10.15886/j.cnki.rdswxb.2011.01.002
Citation: ZHAI Jinling, XU Yuanfeng, MA Hongwei, CHEN Xufeng, HUANG Xi. Cloning and Sequence Analysis of Gene Encoding Vacuolar-type Na+/H+ Antiporter of Rhizophora stylosa（RsNHX1）[J]. Journal of Tropical Biology, 2011, 2(1): 6-13,20. doi: 10.15886/j.cnki.rdswxb.2011.01.002
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Cloning and Sequence Analysis of Gene Encoding Vacuolar-type Na+/H+ Antiporter of Rhizophora stylosa（RsNHX1）

doi: 10.15886/j.cnki.rdswxb.2011.01.002

  • Key Laboratory of Tropic Biological Resources of Ministry of Education/Institute of Bio Science and Technology, Hainan University, Haikou 570228, China

  • Received Date: 2010-12-18
  • Based on consensus sequence of vacuolar-type Na+/H+ antiporter genes（NHX1）,the primers were designed to isolate a cDNA fragment of NHX1 from Rhizophora stylosa by RT-PCR.The full length cDNA was obtained by rapid amplification of cDNA ends（RACE）,and the gene was termed as RsNHX1.This gene is 2 094 bp in length and contains one open reading frame of 1 614 bp,encoding 538 amino acid residues.Accession number of GenBank is FJ627273.Bioinformatics analysis results showed that the amino acid sequence of the RsNHX1 gene is 74% and 69% similar with that of PeNHX1（ABY86892,Populus euphratica） and AtNHX1（AAM34759,Arabidopsis thaliana）,respectively.There are significant differences among these sequences.
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Cloning and Sequence Analysis of Gene Encoding Vacuolar-type Na+/H+ Antiporter of Rhizophora stylosa（RsNHX1）

doi: 10.15886/j.cnki.rdswxb.2011.01.002
  • Key Laboratory of Tropic Biological Resources of Ministry of Education/Institute of Bio Science and Technology, Hainan University, Haikou 570228, China
  • Received Date: 2010-12-18

Abstract: Based on consensus sequence of vacuolar-type Na+/H+ antiporter genes（NHX1）,the primers were designed to isolate a cDNA fragment of NHX1 from Rhizophora stylosa by RT-PCR.The full length cDNA was obtained by rapid amplification of cDNA ends（RACE）,and the gene was termed as RsNHX1.This gene is 2 094 bp in length and contains one open reading frame of 1 614 bp,encoding 538 amino acid residues.Accession number of GenBank is FJ627273.Bioinformatics analysis results showed that the amino acid sequence of the RsNHX1 gene is 74% and 69% similar with that of PeNHX1（ABY86892,Populus euphratica） and AtNHX1（AAM34759,Arabidopsis thaliana）,respectively.There are significant differences among these sequences.

ZHAI Jinling, XU Yuanfeng, MA Hongwei, CHEN Xufeng, HUANG Xi. Cloning and Sequence Analysis of Gene Encoding Vacuolar-type Na+/H+ Antiporter of Rhizophora stylosa（RsNHX1）[J]. Journal of Tropical Biology, 2011, 2(1): 6-13,20. doi: 10.15886/j.cnki.rdswxb.2011.01.002
Citation: ZHAI Jinling, XU Yuanfeng, MA Hongwei, CHEN Xufeng, HUANG Xi. Cloning and Sequence Analysis of Gene Encoding Vacuolar-type Na+/H+ Antiporter of Rhizophora stylosa（RsNHX1）[J]. Journal of Tropical Biology, 2011, 2(1): 6-13,20. doi: 10.15886/j.cnki.rdswxb.2011.01.002
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