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Volume 2 Issue 1
Mar.  2011
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Article Navigation >  Journal of Tropical Biology  > 2011  >  2(1): 1-5

DU Li, XIE Shaolin, CHENG Ying, ZHANG Xiaoru, KUANG Wenhua, JIAO Hanwei, ZHANG Donglin, HAO Yongchang, LEI Ming, LIU Tao, WANG Fengyang. Cloning and Bioinformatics Analysis of MD2 cDNA from Hainan Eld’s deer[J]. Journal of Tropical Biology, 2011, 2(1): 1-5. doi: 10.15886/j.cnki.rdswxb.2011.01.001
Citation: DU Li, XIE Shaolin, CHENG Ying, ZHANG Xiaoru, KUANG Wenhua, JIAO Hanwei, ZHANG Donglin, HAO Yongchang, LEI Ming, LIU Tao, WANG Fengyang. Cloning and Bioinformatics Analysis of MD2 cDNA from Hainan Eld’s deer[J]. Journal of Tropical Biology, 2011, 2(1): 1-5. doi: 10.15886/j.cnki.rdswxb.2011.01.001
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Cloning and Bioinformatics Analysis of MD2 cDNA from Hainan Eld’s deer

doi: 10.15886/j.cnki.rdswxb.2011.01.001

  • College of Agriculture, Hainan University, Haina Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research(Construction Period), Animal Genetic Engineering Key Lab of Haikou, Haikou 570228, China

  • Received Date: 2011-01-16
  • Myeloid differential protein-2（MD2） cDNA of Hainan Eld’s deer（Cervus eldi hainanus） was amplified by RT-PCR and the sequences were analyzed.The bioinformatics analysis results indicated that MD2 cDNA ORF contained a 486 bp encoding 161 amino acids;compared to yellow cattle,human,rabbit and mouse,the nucleotide sequence homology of MD2 were 98.55%,77.43%,78.47% and 74.74%,respectively;relative molecular weight was 19 000,pI was 8.18.The molecular structure prediction results suggested that the protein was non-transmembrane protein,which contained 16.25% α-helix,38.13% β-meander,45.63 % random coil.
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Cloning and Bioinformatics Analysis of MD2 cDNA from Hainan Eld’s deer

doi: 10.15886/j.cnki.rdswxb.2011.01.001
  • College of Agriculture, Hainan University, Haina Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research(Construction Period), Animal Genetic Engineering Key Lab of Haikou, Haikou 570228, China
  • Received Date: 2011-01-16

Abstract: Myeloid differential protein-2（MD2） cDNA of Hainan Eld’s deer（Cervus eldi hainanus） was amplified by RT-PCR and the sequences were analyzed.The bioinformatics analysis results indicated that MD2 cDNA ORF contained a 486 bp encoding 161 amino acids;compared to yellow cattle,human,rabbit and mouse,the nucleotide sequence homology of MD2 were 98.55%,77.43%,78.47% and 74.74%,respectively;relative molecular weight was 19 000,pI was 8.18.The molecular structure prediction results suggested that the protein was non-transmembrane protein,which contained 16.25% α-helix,38.13% β-meander,45.63 % random coil.

DU Li, XIE Shaolin, CHENG Ying, ZHANG Xiaoru, KUANG Wenhua, JIAO Hanwei, ZHANG Donglin, HAO Yongchang, LEI Ming, LIU Tao, WANG Fengyang. Cloning and Bioinformatics Analysis of MD2 cDNA from Hainan Eld’s deer[J]. Journal of Tropical Biology, 2011, 2(1): 1-5. doi: 10.15886/j.cnki.rdswxb.2011.01.001
Citation: DU Li, XIE Shaolin, CHENG Ying, ZHANG Xiaoru, KUANG Wenhua, JIAO Hanwei, ZHANG Donglin, HAO Yongchang, LEI Ming, LIU Tao, WANG Fengyang. Cloning and Bioinformatics Analysis of MD2 cDNA from Hainan Eld’s deer[J]. Journal of Tropical Biology, 2011, 2(1): 1-5. doi: 10.15886/j.cnki.rdswxb.2011.01.001
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