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Volume 1 Issue 4
Dec.  2010
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Article Navigation >  Journal of Tropical Biology  > 2010  >  1(4): 307-313

LI Zhe, DAI Xue-mei, SUN Ai-hua, HUANG Tian-dai, ZHOU Quan-nan, HUANG Hua-sun, LIN Wei-fu, Ludovic Lardet, Pascal Montoro, Marc-Philippe Carron. Nucellus Friable Callus Induction and its Embryoid Ontogeny from Rubber Tree Clone Reyan 88-13[J]. Journal of Tropical Biology, 2010, 1(4): 307-313. doi: 10.15886/j.cnki.rdswxb.2010.04.014
Citation: LI Zhe, DAI Xue-mei, SUN Ai-hua, HUANG Tian-dai, ZHOU Quan-nan, HUANG Hua-sun, LIN Wei-fu, Ludovic Lardet, Pascal Montoro, Marc-Philippe Carron. Nucellus Friable Callus Induction and its Embryoid Ontogeny from Rubber Tree Clone Reyan 88-13[J]. Journal of Tropical Biology, 2010, 1(4): 307-313. doi: 10.15886/j.cnki.rdswxb.2010.04.014
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Nucellus Friable Callus Induction and its Embryoid Ontogeny from Rubber Tree Clone Reyan 88-13

doi: 10.15886/j.cnki.rdswxb.2010.04.014

  • 1. Rubber Research Institute, CATAS; Key Laboratory of Rubber Biology, Ministry of Agriculture; State Engineering and TechnologyResearch Center for Key Tropical Crops, Danzhou 571737, China;

  • 2. UMR-1098 Biologie du développement des Espèces Pérennes, CIRAD, TA 80/03, Avenue d'Agropolis, Montpellier Cedex 5 France 34398

  • Received Date: 2010-08-20
  • The nucellar tissue from the young seeds of rubber tree clone Reyan 88-13 was used to induce embryogenic callus. The different culture parameters such as the different types and concentrations of plant growth regulators and different calcium concentrations in embryogenic callus induction media and subculture media had been analyzed and the optimal culture parameters had been determined. Through continuous subculture and selection for 5 months,friable embryogenic callus had been gradually obtained. Long term subculture had been carried on for the friable embryogenic calli. Histological sections proved that the calli proliferated by long term subculture maintained the embryogenic characters. The nucellar friable and embryogenic calli of rubber tree clone Reyan 88-13 that had been subcultured for more than 2 years were used for inducing embryoids,and 186 embryoids were obtained,which were being used to induce plant regeneration.
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Nucellus Friable Callus Induction and its Embryoid Ontogeny from Rubber Tree Clone Reyan 88-13

doi: 10.15886/j.cnki.rdswxb.2010.04.014
  • 1. Rubber Research Institute, CATAS; Key Laboratory of Rubber Biology, Ministry of Agriculture; State Engineering and TechnologyResearch Center for Key Tropical Crops, Danzhou 571737, China;
  • 2. UMR-1098 Biologie du développement des Espèces Pérennes, CIRAD, TA 80/03, Avenue d'Agropolis, Montpellier Cedex 5 France 34398
  • Received Date: 2010-08-20

Abstract: The nucellar tissue from the young seeds of rubber tree clone Reyan 88-13 was used to induce embryogenic callus. The different culture parameters such as the different types and concentrations of plant growth regulators and different calcium concentrations in embryogenic callus induction media and subculture media had been analyzed and the optimal culture parameters had been determined. Through continuous subculture and selection for 5 months,friable embryogenic callus had been gradually obtained. Long term subculture had been carried on for the friable embryogenic calli. Histological sections proved that the calli proliferated by long term subculture maintained the embryogenic characters. The nucellar friable and embryogenic calli of rubber tree clone Reyan 88-13 that had been subcultured for more than 2 years were used for inducing embryoids,and 186 embryoids were obtained,which were being used to induce plant regeneration.

LI Zhe, DAI Xue-mei, SUN Ai-hua, HUANG Tian-dai, ZHOU Quan-nan, HUANG Hua-sun, LIN Wei-fu, Ludovic Lardet, Pascal Montoro, Marc-Philippe Carron. Nucellus Friable Callus Induction and its Embryoid Ontogeny from Rubber Tree Clone Reyan 88-13[J]. Journal of Tropical Biology, 2010, 1(4): 307-313. doi: 10.15886/j.cnki.rdswxb.2010.04.014
Citation: LI Zhe, DAI Xue-mei, SUN Ai-hua, HUANG Tian-dai, ZHOU Quan-nan, HUANG Hua-sun, LIN Wei-fu, Ludovic Lardet, Pascal Montoro, Marc-Philippe Carron. Nucellus Friable Callus Induction and its Embryoid Ontogeny from Rubber Tree Clone Reyan 88-13[J]. Journal of Tropical Biology, 2010, 1(4): 307-313. doi: 10.15886/j.cnki.rdswxb.2010.04.014
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