Advance Search

Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review, editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name
E-mail
Phone
Title
Content
Verification Code
Volume 1 Issue 4
Dec.  2010
Turn off MathJax
Article Contents
Article Navigation >  Journal of Tropical Biology  > 2010  >  1(4): 337-341

XIE Jun, ZHANG Xu-yi, CHEN Yin-hua, CHEN Cai-yan, WEI Shuang-shuang, XIA You-quan, TANG Hua. Isolation of Fusarium oxysporum f.sp.cubense and Its Morphological Investigation[J]. Journal of Tropical Biology, 2010, 1(4): 337-341. doi: 10.15886/j.cnki.rdswxb.2010.04.010
Citation: XIE Jun, ZHANG Xu-yi, CHEN Yin-hua, CHEN Cai-yan, WEI Shuang-shuang, XIA You-quan, TANG Hua. Isolation of Fusarium oxysporum f.sp.cubense and Its Morphological Investigation[J]. Journal of Tropical Biology, 2010, 1(4): 337-341. doi: 10.15886/j.cnki.rdswxb.2010.04.010
shu

Isolation of Fusarium oxysporum f.sp.cubense and Its Morphological Investigation

doi: 10.15886/j.cnki.rdswxb.2010.04.010

  • 1. College of Agriculture, Hainan University, Haikou 570228, China;

  • 2. National Key Laboratory Base of Tropic Biological Resources Sustainable Utilization, Hainan University, Haikou 570228, China;

  • 3. Agricultural Technology Popularizing Center of Haikou County, Haikou 570206, China

  • Received Date: 2010-09-07
  • Fusarium wilt of banana is a destructive fungus disease caused by Fusarium oxysporum f.sp.Cubense,and which is serious harm to banana planting and its industry development. In order to study the pathogenic mechanism of the disease and the resistance mechanism of banana,tissue isolation method was used to isolate and purify the candidate fungus strain,which were observed by morphological method; CTAB method was used to extract DNA of fungus,and ITS （Internal Transcribed Spacers） sequence was amplified and analyzed; the pathogenic fungus were identified by molecular method. The results indicated that the candidate strain was Fusarium oxysporum f.sp.Cubense.,and which was confirmed as pathogenic fungi of banana wilt disease.
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索
Get Citation
PDF
XML

Article views(23) PDF downloads(0) Cited by()

Proportional views
Related

Isolation of Fusarium oxysporum f.sp.cubense and Its Morphological Investigation

doi: 10.15886/j.cnki.rdswxb.2010.04.010
  • 1. College of Agriculture, Hainan University, Haikou 570228, China;
  • 2. National Key Laboratory Base of Tropic Biological Resources Sustainable Utilization, Hainan University, Haikou 570228, China;
  • 3. Agricultural Technology Popularizing Center of Haikou County, Haikou 570206, China
  • Received Date: 2010-09-07

Abstract: Fusarium wilt of banana is a destructive fungus disease caused by Fusarium oxysporum f.sp.Cubense,and which is serious harm to banana planting and its industry development. In order to study the pathogenic mechanism of the disease and the resistance mechanism of banana,tissue isolation method was used to isolate and purify the candidate fungus strain,which were observed by morphological method; CTAB method was used to extract DNA of fungus,and ITS （Internal Transcribed Spacers） sequence was amplified and analyzed; the pathogenic fungus were identified by molecular method. The results indicated that the candidate strain was Fusarium oxysporum f.sp.Cubense.,and which was confirmed as pathogenic fungi of banana wilt disease.

XIE Jun, ZHANG Xu-yi, CHEN Yin-hua, CHEN Cai-yan, WEI Shuang-shuang, XIA You-quan, TANG Hua. Isolation of Fusarium oxysporum f.sp.cubense and Its Morphological Investigation[J]. Journal of Tropical Biology, 2010, 1(4): 337-341. doi: 10.15886/j.cnki.rdswxb.2010.04.010
Citation: XIE Jun, ZHANG Xu-yi, CHEN Yin-hua, CHEN Cai-yan, WEI Shuang-shuang, XIA You-quan, TANG Hua. Isolation of Fusarium oxysporum f.sp.cubense and Its Morphological Investigation[J]. Journal of Tropical Biology, 2010, 1(4): 337-341. doi: 10.15886/j.cnki.rdswxb.2010.04.010
shu

    HTML

Catalog

    Copyright © 《Journal of Tropical Biology》Editorial Board

    Address: 58 Renmin Avenue, Haikou, Hainan China Pos: 100101Tel: 0898-66281595

    Supported by: Beijing Renhe Information Technology Co. Ltd

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return