Impact of <i>Calonectria</i> leaf blight on soil fungal <i>diversity</i> under Eucalyptus spp. Forests

WEI Hai; LI Changtao; MO Qijin; LI Wen; GUAN Wei; LUO Changming; LUO Dun; ZHONG Minglong

doi:10.15886/j.cnki.rdswxb.20240102

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Volume 16 Issue 2

Apr. 2025

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Article Navigation > Journal of Tropical Biology > 2025 > 16(2): 227-235

WEI Hai, LI Changtao, MO Qijin, LI Wen, GUAN Wei, LUO Changming, LUO Dun, ZHONG Minglong. Impact of Calonectria leaf blight on soil fungal diversity under Eucalyptus spp. Forests[J]. Journal of Tropical Biology, 2025, 16(2): 227-235. doi: 10.15886/j.cnki.rdswxb.20240102

Citation:

WEI Hai, LI Changtao, MO Qijin, LI Wen, GUAN Wei, LUO Changming, LUO Dun, ZHONG Minglong. Impact of Calonectria leaf blight on soil fungal diversity under Eucalyptus spp. Forests[J]. Journal of Tropical Biology, 2025, 16(2): 227-235. doi: 10.15886/j.cnki.rdswxb.20240102

Impact of Calonectria leaf blight on soil fungal diversity under Eucalyptus spp. Forests

doi: 10.15886/j.cnki.rdswxb.20240102

State-owned Bobai Forest Farm of Guangxi Zhuang Autonomous Region, Yulin, Guangxi 537600, China

Received Date: 2024-06-21
Rev Recd Date: 2024-08-02

Abstract

An attempt was made to compare and analyze the composition and structure of soil fungal communities under Eucalyptus spp. stands healthy or infected with Calonectria leaf blight based on Illumina high-throughput sequencing so as to reveal the interactions between the pathogen Calonectria and other fungi, which might provide theoretical support for the development of biocontrol agents. A total of 2 817 fungal ASVs（Amplicon Sequence Variants） were annotated from 12 tested samples, classified into 12 phyla, 421 genera, and 614 species.There was no significant difference in alpha diversity of soil fungi between the diseased and healthy Eucalyptus spp. stands, with dominant phyla being consistent. Among the top five most abundant genera, the genera with the highest abundance were unclassified＿k＿Fungi followed by Saitozyma, while the other three genera differed between the two groups. Differential analysis of fungal abundances and correlation analysis with the pathogen Calonectria showed that Thelephora ranked fifth in abundance in healthy stands and had significantly higher levels in healthy stand soils compared to the diseased stands, suggesting it could be an antagonist of Calonectria leaf blight. Temitomyces and Xyladictyochaeta both had significantly higher abundances in the diseased stands than in the healthy ones（P ＜ 0.05） and could serve as potential biocontrol agents. Calonectria had a negative correlation with Ophiocordyceps, which is commonly used as a biocontrol agent and could also be a candidate for controlling Calonectria leaf blight. The findings revealed that the Calonectria leaf blight pathogen Calonectria comprised a relatively small proportion of the fungal community in Eucalyptus spp. soils but still had the pathogenicity to cause disease in Eucalyptus spp. These results provided theoretical support for the development of biocontrol agents and antagonists against Calonectria leaf blight in Eucalyptus spp.
- Eucalyptus spp.,
- Calonectria leaf blight,
- fungi,
- high-throughput sequencing

References

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[2]	韦月越,蒙敏,黄雪芬,等.桉树对矿区污染土壤中Cu和Cd的耐受机制[J]. 基因组学与应用生物学, 2016,35(1):227-234.
[3]	黄国勤,赵其国.广西桉树种植的历史、现状、生态问题及应对策略[J]. 生态学报, 2014, 34(18):5142-5152.
[4]	任一平,任世奇,朱慧,等.桉树人工林碳储量的研究方法及影响因素[J]. 桉树科技, 2023, 40(3):94-100.
[5]	朱宇林,何斌,杨钙仁,等.尾巨桉人工林营养元素积累及其生物循环特征[J]. 东北林业大学学报, 2012, 40(6):8-11.
[6]	王东亮.广东省木材加工业发展影响因素研究[D]. 北京:北京林业大学, 2013.
[7]	叶小真.桉树焦枯病菌全基因组测序及致病相关基因分析[D]. 福州:福建农林大学, 2016.
[8]	黄秀萍,冯丽贞,李庭波,等.基于文献计量的桉树焦枯病研究现状[J]. 森林与环境学报, 2018, 38(3):375-384.
[9]	李贵玉.广西桉树病虫害发生现状及防治策略[J]. 广西林业科学, 2006, 35(4):285-288.
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[13]	李晶,刘玉荣,贺纪正,等.土壤微生物对环境胁迫的响应机制[J]. 环境科学学报, 2013, 33(4):959-967.
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[19]	LUTSIV T, WEIR T L, MCGINLEY J N, et al. Compositional changes of the high-fat diet-Induced gut microbiota upon consumption of common pulses[J]. Nutrients,2021, 13(11):3992.
[20]	LI M, MI T, HE H, et al. Active bacterial and archaeal communities in coastal sediments:Biogeography pattern,assembly process and co-occurrence relationship[J]. The Science of the Total Environment, 2021, 750:142252.
[21]	林先贵,胡君利.土壤微生物多样性的科学内涵及其生态服务功能[J]. 土壤学报, 2008, 45(5):892-900.
[22]	沈宏,曹志洪,胡正义.土壤活性有机碳的表征及其生态效应[J]. 生态学杂志, 1999(3):33-39.
[23]	司伟壮.山核桃根腐病发生与生境微生物菌群消长的关系[D]. 杭州:浙江农林大学, 2021.
[24]	贺璐,马杰,傅淋,等.卷丹百合根腐病植株与健康植株根际土壤真菌群落结构差异[J]. 西南农业学报,2023, 36(11):2419-2425.
[25]	樊俊,谭军,向必坤,等.雪茄烟根腐病与土壤真菌群落和理化性状的相关性分析[J]. 中国烟草科学, 2023,44(1):100-106.
[26]	甄雪冰.土传病害生防菌株的筛选鉴定及防治效果研究[D]. 南京:南京林业大学, 2009.
[27]	李娅,朱潇逸,景跃波,等.西南桦苗木猝倒病生防菌的筛选及鉴定研究[J]. 西部林业科学, 2013, 42(6):109-114.
[28]	管鸿智,黄荣珍,王金平,等.红壤区退化林地表土真菌群落结构对土壤改良措施的响应[J]. 环境科学,2023, 44(1):494-501.
[29]	郭洁,樊竹青,赵利坤,等.普洱地区桉树林根际土壤真菌群落结构和多样性研究[J]. 普洱学院学报, 2022,38(6):23-26.
[30]	韦菊娴,王聪,何斌,等.世界桉树林土壤微生物研究综述[J]. 浙江农林大学学报, 2022, 39(5):1144-1154.
[31]	GUO H, HE T, LEE D J. Contemporary proteomic research on lignocellulosic enzymes and enzymolysis:A review[J]. Bioresource Technology, 2022, 334(Pt B):126263.
[32]	WIRIYA J, KAVINLERTVATANA P, LUMYONG S.Effects of different culture media, carbon and nitrogen sources and solid substrates on growth of Termitomyces mushrooms[J]. Chiang Mai Journal of Science, 2014,41(3):542-556.
[33]	HEREDIA G, LI D W, WENDT L, et al. Natonodosa speciosa gen. et sp. nov. and rediscovery of Poroisariopsis inornata:neotropical anamorphic fungi in Xylariales[J]. Mycological Progress, 2020,19(1):15-30.
[34]	LU J, SU X, YANG Z, et al. The correlation between the gut microbiota of Endoclita signifier(Lepidoptera, Hepialidae) larvae and their host preferences[J]. Insects,2023, 14(12):919.
[35]	吕开源,周立萍,康建宏,等.不同耕作方式下玉米秸秆还田对土壤真菌群落的影响[J]. 中国土壤与肥料,2022(8):112-122.
[36]	罗基同,薛振南,廖旺姣,等.广西速生丰产桉树病害调查[J]. 中国森林病虫, 2012, 31(4):21-24.
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[38]	丘雪红,曹莉,韩日畴.冬虫夏草的研究进展、现存问题与研究展望[J]. 环境昆虫学报, 2016, 38(1):1-23.
[39]	刘艳娇,樊丹丹,李香真,等.人工与天然云杉林土壤真菌群落多样性及菌群网络关系特征[J]. 应用生态学报, 2021, 32(4):1441-1451.
[40]	周杰,谢泰祥,刘江枫,等.野生建兰根系共生真菌群落结构及生物学功能[J]. 微生物学报, 2021, 61(7):2136-2153.
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[46]	许红丽,王树强,张玉兰,等.菌糠源生物堆肥对黄瓜种植土壤微生物活性、数量和多样性的影响[J]. 生态学杂志, 2024, 43(5):1279-1290.

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Impact of Calonectria leaf blight on soil fungal diversity under Eucalyptus spp. Forests

State-owned Bobai Forest Farm of Guangxi Zhuang Autonomous Region, Yulin, Guangxi 537600, China

Received Date: 2024-06-21

Rev Recd Date: 2024-08-02

Key words:

Abstract: An attempt was made to compare and analyze the composition and structure of soil fungal communities under Eucalyptus spp. stands healthy or infected with Calonectria leaf blight based on Illumina high-throughput sequencing so as to reveal the interactions between the pathogen Calonectria and other fungi, which might provide theoretical support for the development of biocontrol agents. A total of 2 817 fungal ASVs（Amplicon Sequence Variants） were annotated from 12 tested samples, classified into 12 phyla, 421 genera, and 614 species.There was no significant difference in alpha diversity of soil fungi between the diseased and healthy Eucalyptus spp. stands, with dominant phyla being consistent. Among the top five most abundant genera, the genera with the highest abundance were unclassified＿k＿Fungi followed by Saitozyma, while the other three genera differed between the two groups. Differential analysis of fungal abundances and correlation analysis with the pathogen Calonectria showed that Thelephora ranked fifth in abundance in healthy stands and had significantly higher levels in healthy stand soils compared to the diseased stands, suggesting it could be an antagonist of Calonectria leaf blight. Temitomyces and Xyladictyochaeta both had significantly higher abundances in the diseased stands than in the healthy ones（P ＜ 0.05） and could serve as potential biocontrol agents. Calonectria had a negative correlation with Ophiocordyceps, which is commonly used as a biocontrol agent and could also be a candidate for controlling Calonectria leaf blight. The findings revealed that the Calonectria leaf blight pathogen Calonectria comprised a relatively small proportion of the fungal community in Eucalyptus spp. soils but still had the pathogenicity to cause disease in Eucalyptus spp. These results provided theoretical support for the development of biocontrol agents and antagonists against Calonectria leaf blight in Eucalyptus spp.

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Reference (46)

[1]	谢耀坚.中国桉树人工林可持续经营战略初探[J]. 世界林业研究, 2003, 16(5):59-64.
[2]	韦月越,蒙敏,黄雪芬,等.桉树对矿区污染土壤中Cu和Cd的耐受机制[J]. 基因组学与应用生物学, 2016,35(1):227-234.
[3]	黄国勤,赵其国.广西桉树种植的历史、现状、生态问题及应对策略[J]. 生态学报, 2014, 34(18):5142-5152.
[4]	任一平,任世奇,朱慧,等.桉树人工林碳储量的研究方法及影响因素[J]. 桉树科技, 2023, 40(3):94-100.
[5]	朱宇林,何斌,杨钙仁,等.尾巨桉人工林营养元素积累及其生物循环特征[J]. 东北林业大学学报, 2012, 40(6):8-11.
[6]	王东亮.广东省木材加工业发展影响因素研究[D]. 北京:北京林业大学, 2013.
[7]	叶小真.桉树焦枯病菌全基因组测序及致病相关基因分析[D]. 福州:福建农林大学, 2016.
[8]	黄秀萍,冯丽贞,李庭波,等.基于文献计量的桉树焦枯病研究现状[J]. 森林与环境学报, 2018, 38(3):375-384.
[9]	李贵玉.广西桉树病虫害发生现状及防治策略[J]. 广西林业科学, 2006, 35(4):285-288.
[10]	陈全助,郭文硕,叶小真,等.福建省桉树焦枯病菌分类鉴定[J]. 福建林学院学报, 2013, 33(2):176-182.
[11]	陈帅飞.中国桉树真菌病原汇录:2006-2013[J]. 桉树科技, 2014, 31(1):37-65.
[12]	徐阳春,沈其荣,冉炜.长期免耕与施用有机肥对土壤微生物生物量碳、氮、磷的影响[J]. 土壤学报, 2002,39(1):89-96.
[13]	李晶,刘玉荣,贺纪正,等.土壤微生物对环境胁迫的响应机制[J]. 环境科学学报, 2013, 33(4):959-967.
[14]	陈剑山,郑服丛. ITS序列分析在真菌分类鉴定中的应用[J]. 安徽农业科学, 2007, 35(13):3785-3786.
[15]	CHEN S, ZHOU Y, CHEN Y, et al. Fastp:an ultra-fast all-in-one FASTQ preprocessor[J]. Bioinformatics, 2018,34(17):i884-i890.
[16]	MAGOČT, SALZBERG S L. FLASH:fast length adjustment of short reads to improve genome assemblies[J]. Bioinformatics, 2011, 27(21):2957-2963.
[17]	ZHANG H, CARNEVALE G, POLESE B, et al. CD109restrains activation of cutaneous IL-17-producing γδT cells by commensal microbiota[J]. Cell Reports, 2019,29(2):391-405.e5.
[18]	ALESSANDRI G, MILANI C, MANCABELLI L, et al.Metagenomic dissection of the canine gut microbiota:insights into taxonomic, metabolic and nutritional features[J]. Environmental Microbiology, 2019, 21(4):1331-1343.
[19]	LUTSIV T, WEIR T L, MCGINLEY J N, et al. Compositional changes of the high-fat diet-Induced gut microbiota upon consumption of common pulses[J]. Nutrients,2021, 13(11):3992.
[20]	LI M, MI T, HE H, et al. Active bacterial and archaeal communities in coastal sediments:Biogeography pattern,assembly process and co-occurrence relationship[J]. The Science of the Total Environment, 2021, 750:142252.
[21]	林先贵,胡君利.土壤微生物多样性的科学内涵及其生态服务功能[J]. 土壤学报, 2008, 45(5):892-900.
[22]	沈宏,曹志洪,胡正义.土壤活性有机碳的表征及其生态效应[J]. 生态学杂志, 1999(3):33-39.
[23]	司伟壮.山核桃根腐病发生与生境微生物菌群消长的关系[D]. 杭州:浙江农林大学, 2021.
[24]	贺璐,马杰,傅淋,等.卷丹百合根腐病植株与健康植株根际土壤真菌群落结构差异[J]. 西南农业学报,2023, 36(11):2419-2425.
[25]	樊俊,谭军,向必坤,等.雪茄烟根腐病与土壤真菌群落和理化性状的相关性分析[J]. 中国烟草科学, 2023,44(1):100-106.
[26]	甄雪冰.土传病害生防菌株的筛选鉴定及防治效果研究[D]. 南京:南京林业大学, 2009.
[27]	李娅,朱潇逸,景跃波,等.西南桦苗木猝倒病生防菌的筛选及鉴定研究[J]. 西部林业科学, 2013, 42(6):109-114.
[28]	管鸿智,黄荣珍,王金平,等.红壤区退化林地表土真菌群落结构对土壤改良措施的响应[J]. 环境科学,2023, 44(1):494-501.
[29]	郭洁,樊竹青,赵利坤,等.普洱地区桉树林根际土壤真菌群落结构和多样性研究[J]. 普洱学院学报, 2022,38(6):23-26.
[30]	韦菊娴,王聪,何斌,等.世界桉树林土壤微生物研究综述[J]. 浙江农林大学学报, 2022, 39(5):1144-1154.
[31]	GUO H, HE T, LEE D J. Contemporary proteomic research on lignocellulosic enzymes and enzymolysis:A review[J]. Bioresource Technology, 2022, 334(Pt B):126263.
[32]	WIRIYA J, KAVINLERTVATANA P, LUMYONG S.Effects of different culture media, carbon and nitrogen sources and solid substrates on growth of Termitomyces mushrooms[J]. Chiang Mai Journal of Science, 2014,41(3):542-556.
[33]	HEREDIA G, LI D W, WENDT L, et al. Natonodosa speciosa gen. et sp. nov. and rediscovery of Poroisariopsis inornata:neotropical anamorphic fungi in Xylariales[J]. Mycological Progress, 2020,19(1):15-30.
[34]	LU J, SU X, YANG Z, et al. The correlation between the gut microbiota of Endoclita signifier(Lepidoptera, Hepialidae) larvae and their host preferences[J]. Insects,2023, 14(12):919.
[35]	吕开源,周立萍,康建宏,等.不同耕作方式下玉米秸秆还田对土壤真菌群落的影响[J]. 中国土壤与肥料,2022(8):112-122.
[36]	罗基同,薛振南,廖旺姣,等.广西速生丰产桉树病害调查[J]. 中国森林病虫, 2012, 31(4):21-24.
[37]	王淑玉.不同地区烟株根际土壤微生态变化分析[D]. 郑州:郑州大学, 2015.
[38]	丘雪红,曹莉,韩日畴.冬虫夏草的研究进展、现存问题与研究展望[J]. 环境昆虫学报, 2016, 38(1):1-23.
[39]	刘艳娇,樊丹丹,李香真,等.人工与天然云杉林土壤真菌群落多样性及菌群网络关系特征[J]. 应用生态学报, 2021, 32(4):1441-1451.
[40]	周杰,谢泰祥,刘江枫,等.野生建兰根系共生真菌群落结构及生物学功能[J]. 微生物学报, 2021, 61(7):2136-2153.
[41]
[42]	余著成.伯乐树根际土壤肥力状况和微生物群落特征及其影响因素研究[D]. 杭州:浙江农林大学, 2021.
[43]	谭小明,周雅琴,陈娟,等.药用植物内生真菌多样性研究进展[J]. 中国药学杂志, 2015, 50(18):1563-1580.
[44]
[45]	蔡媛,刘浩,孔文平,等.多花黄精内生菌群落结构多样性及其与有效成分含量相关性研究[J]. 中草药,2021, 52(13):4023-4030.
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Impact of Calonectria leaf blight on soil fungal diversity under Eucalyptus spp. Forests

doi: 10.15886/j.cnki.rdswxb.20240102

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Impact of Calonectria leaf blight on soil fungal diversity under Eucalyptus spp. Forests

doi: 10.15886/j.cnki.rdswxb.20240102

State-owned Bobai Forest Farm of Guangxi Zhuang Autonomous Region, Yulin, Guangxi 537600, China

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Impact of Calonectria leaf blight on soil fungal diversity under Eucalyptus spp. Forests

doi: 10.15886/j.cnki.rdswxb.20240102

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Impact of Calonectria leaf blight on soil fungal diversity under Eucalyptus spp. Forests

doi: 10.15886/j.cnki.rdswxb.20240102

State-owned Bobai Forest Farm of Guangxi Zhuang Autonomous Region, Yulin, Guangxi 537600, China

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