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Volume 16 Issue 2
Apr.  2025
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Article Navigation >  Journal of Tropical Biology  > 2025  >  16(2): 243-253

LIU Huiyao, LUO Tianxiong, WANG Ling, YANG Fusun. Investigation of the natural symbiotic relationship between mycorrhizal fungi and endophytic bacteria in Dendrobium nobile[J]. Journal of Tropical Biology, 2025, 16(2): 243-253. doi: 10.15886/j.cnki.rdswxb.20240098
Citation: LIU Huiyao, LUO Tianxiong, WANG Ling, YANG Fusun. Investigation of the natural symbiotic relationship between mycorrhizal fungi and endophytic bacteria in Dendrobium nobile[J]. Journal of Tropical Biology, 2025, 16(2): 243-253. doi: 10.15886/j.cnki.rdswxb.20240098
shu

Investigation of the natural symbiotic relationship between mycorrhizal fungi and endophytic bacteria in Dendrobium nobile

doi: 10.15886/j.cnki.rdswxb.20240098

  • School of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China

  • Received Date: 2024-06-13
  • Rev Recd Date: 2024-08-14
  • An attempt was made to investigate the naturally occurring, difficult-to-separate combinations of mycorrhizal fungi and endophytic bacteria found in the mycorrhizae of Dendrobium nobile Lindl. Three groups of artificially cultivable and separable natural combinations of mycorrhizal fungi and endophytic bacteria were isolated,screened, and identified. Through co-cultivation experiments and functional assessments, the symbiotic relationships and mechanisms among these combinations were analyzed. The results showed that G2 and G8 are symbiotic combinations of Bacillus and Fusarium solani, while G17 is a combination of Bacillus and Diaporthe phaseolorum.There is an antagonistic relationship between the G2 and G17 combinations, leading to an increased total growth,whereas the G8 combination exhibits no antagonistic relationship, with mutual promotion between the two. The fungi in the G2 and G17 combinations possess strong cellulolytic abilities, with clear zone diameters of 27.62 mm and 21.52 mm,respectively, while the bacteria exhibit nitrogen-fixing abilities of 1.69 μg·mL-1 and 1.68 μg·mL-1, respectively. The strong cellulolytic and nitrogen-fixing abilities contribute to the antagonistic relationship between the G2 and G17combinations. In the G8 combination, the fungal solution contains the lowest hydrolyzed phosphorus, while the bacteria have the highest phosphorus-solubilizing ability, reaching 39.07 mg·kg-1, indicating a complementary functional relationship. Therefore, natural symbiotic combinations exhibit various forms of symbiotic relationships, including complementarity, promotion, and inhibition, and there is a correlation between function and symbiotic relationship.
  • [1] DUPONNOIS R, GARBAYE J. Mycorrhization helper bacteria associated with the douglas fir-laccaria laccata symbiosis:effects in aseptic and in glasshouse conditions[J]. Annales des Sciences Forestières, 1991, 48(3):239-251.
    [2] FITTER HA, GARBAYE J. Interactions between mycorrhizal fungi and other soil organisms[J]. Plant and Soil,1994, 159(1):123-132.
    [3] POOLE EJ, BENDING GD, WHIPPS JM, et al. Bacteria associated with Pinus sylvestris-Lactarius rufus ectomycorrhizas and their effects on mycorrhiza formation in vitro[J]. The New Phytologist, 2001, 151(3):743-751.
    [4] DUPONNOIS R. Bacteria helping mycorrhiza development[M]. Microbial Activity in the Rhizoshere, Berlin, Heidelberg:Springer, 2006:297-310.
    [5] ZENG Yingjie, YANG Huirong, WU Xiaoling, et al. Structure and immunomodulatory activity of polysaccharides from Fusarium solani do7 by solid-state fermentation[J]. International Journal of Biological Macromolecules, 2019,137:568-575.
    [6] JIN Zhaoxia, LI Dandan, LIU Tianyi, et al. Cultural endophytic fungi associated with Dendrobium officinale identification, diversity estimation and their antimicrobial potential[J]. Current Science, 2017, 112(8):1690-1690.
    [7] 张萍.铁皮石斛内生细菌研究[D]. 海口:海南大学, 2012.
    [8] 国家药典委员会.中华人民共和国药典[M]. 第一部.北京:中国医药科技出版社, 2015:92.
    [9] 王凤忠,王东晖.石斛[M]. 北京:中国农业科学技术出版社. 2015:127.
    [10] 张智,林秀莲,李璐,等.惠州龙眼树上铁皮石斛仿生态栽培技术[J]. 园艺与种苗, 2021, 41(02):10-11.
    [11] 罗天雄,杨福孙.石斛附生宿主槟榔微生物分离鉴定及优势真菌拮抗关系[J/OL]. 分子植物育种, 2023:1-18.
    [12] 王晓鸣.海南特有种华石斛菌根生物学研究[D]. 海口:海南大学, 2017.
    [13] 安忠琦.金钗石斛内生细菌的分离及抑菌活性研究[D]. 贵阳:贵州师范大学, 2017.
    [14] HAM SH, YOON AR, OH HE, PARK YG. Plant GrowthPromoting microorganism Pseudarthrobacter sp.NIBRBAC000502770 nhances the growth and flavonoid content of geum aleppicum[J]. Microorganisms. 2022;10(6):1241.
    [15] 索云凯.解钾菌的分离筛选及对水稻田土壤钾释放研究[D]. 大庆:东北石油大学, 2021.
    [16] PARK JH, BOLAN N, MEGHARAJ M, NAIDU R. Concomitant rock phosphate dissolution and lead immobilization by phosphate solubilizing bacteria(Enterobacter sp.)[J]. Journal of Environmental Management. 2011 Apr; 92(4):1115-20.
    [17] YOON, JJ and KIM YK.“Degradation of crystalline cellulose by the brown-rot basidiomycete Fomitopsis palustris.” Journal of microbiology, 2005, 436:487-92.
    [18] 焦振泉,刘秀梅. 16s rRNA序列同源性分析与细菌系统分类鉴定[J]. 国外医学(卫生学分册), 1998(01):14

    -18.
    [19] GLASS N L, DONALDSON G C. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes[J]. Applied and Environmental Microbiology, 1995, 61(4):1323-1330.
    [20] 胡亚强.新蚜虫疠霉真菌共生细菌及其互作研究[D]. 杭州:中国计量大学, 2018.
    [21] 路福平,李玉.微生物学实验技术[M]. 中国轻工业出版社, 2020:73.
    [22] 马敏芝.黑麦草内生真菌生物学、生理学及其抑菌特性研究[D]. 兰州:兰州大学, 2009.
    [23] 尚晓静,侯瑞,徐芳玲,等. 2株蓝莓溶磷内生真菌的筛选、鉴定及溶磷效果评价[J]. 江苏农业科学, 2022,50(20):246-252.
    [24] 王笠,沈建华.混合涂片革兰氏染色法鉴别细菌[J]. 科技创新导报, 2018, 15(24):79+81.
    [25] 乔志伟,张永杰,谢梅芳.黔中黄壤溶磷细菌溶磷特性及对碳循环基因的影响[J/OL]. 河南农业科学:1-11,2023.
    [26] 孙建光,张燕春,徐晶,等.高效固氮芽孢杆菌筛选及其生物学特性[J]. 中国农业科学, 2009, 42(06):2043-2051.
    [27] 盛下放,黄为一.硅酸盐细菌NBT菌株解钾机理初探[J]. 土壤学报, 2002(06):863-871.
    [28] CANTWELL BA, MCCONNELL DJ. Molecular cloning and expression of a Bacillus subtilis β-glucanase gene in Escherichia coli[J]. Gene, 1983, 23(2):211-219.
    [29] 姜晓宇,高菊生,徐凤花,等.水稻种子内生细菌多样性及其分泌植物生长素能力的测定[J]. 微生物学报,2013, 53(3):269-275.
    [30] 苗永美,苗翠苹,于庆才.枯草芽孢杆菌BBs-27发酵液性质及脂肽对黄色镰刀菌的抑菌作用[J]. 生物技术通报, 2023, 39(9):255-267.
    [31] 桑建伟,杨扬,陈奕鹏,等.内生解淀粉芽孢杆菌BEB17脂肽类和聚酮类化合物的抑菌活性分析[J]. 植物病理学报, 2018, 48(3):402-412.
    [32] 杨晓燕,王钰鑫,叶伟伟,等.枯草芽孢杆菌对几种植物病原真菌的抑菌活性[J]. 工业微生物, 2018, 48(6):32-38.
    [33] 陈相波.新蚜虫疠霉(虫霉目)共生细菌多样性研究[D]. 杭州:中国计量学院, 2014.
    [34] XU HB, TSUKUDA M, TAKAHARA Y, et al. Lithoautotrophical oxidation of elemental sulfur by fungi including Fusarium solani isolated from sandstone Angkor temples[J/OL]. International Biodeterioration&Biodegradation,126, 2018:95-102.
    [35] WALLEY FL, GERMIDA JJ. Failure to decontaminate Glomus clarum NT4 spores is due to spore wallassociated bacteria[J]. Mycorrhiza, 1995,6(1):43-49.
    [36] XAVIER LJC, GERMIDA JJ. Bacteria associated with Glomus clarum spores influence mycorrhizal activity[J]. Soil Biology and Biochemistry, 2023, 35(3):471-478.
    [37] PAKVAZ S, SOLTANI J. Endohyphal bacteria from fungal endophytes of the Mediterranean cypress(Cupressus sempervirens) exhibit in vitro bioactivity[J]. Forest Pathology, 2016, 46(6):569-581.
    [38]
    [39] 黄挺,党俊杰,陈洪满,等.生物有机肥中解磷菌和固氮菌的筛选与鉴定[J]. 广西科技大学学报, 2023,34(1):92-97

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Investigation of the natural symbiotic relationship between mycorrhizal fungi and endophytic bacteria in Dendrobium nobile

doi: 10.15886/j.cnki.rdswxb.20240098
  • School of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China
  • Received Date: 2024-06-13
  • Rev Recd Date: 2024-08-14

Abstract: An attempt was made to investigate the naturally occurring, difficult-to-separate combinations of mycorrhizal fungi and endophytic bacteria found in the mycorrhizae of Dendrobium nobile Lindl. Three groups of artificially cultivable and separable natural combinations of mycorrhizal fungi and endophytic bacteria were isolated,screened, and identified. Through co-cultivation experiments and functional assessments, the symbiotic relationships and mechanisms among these combinations were analyzed. The results showed that G2 and G8 are symbiotic combinations of Bacillus and Fusarium solani, while G17 is a combination of Bacillus and Diaporthe phaseolorum.There is an antagonistic relationship between the G2 and G17 combinations, leading to an increased total growth,whereas the G8 combination exhibits no antagonistic relationship, with mutual promotion between the two. The fungi in the G2 and G17 combinations possess strong cellulolytic abilities, with clear zone diameters of 27.62 mm and 21.52 mm,respectively, while the bacteria exhibit nitrogen-fixing abilities of 1.69 μg·mL-1 and 1.68 μg·mL-1, respectively. The strong cellulolytic and nitrogen-fixing abilities contribute to the antagonistic relationship between the G2 and G17combinations. In the G8 combination, the fungal solution contains the lowest hydrolyzed phosphorus, while the bacteria have the highest phosphorus-solubilizing ability, reaching 39.07 mg·kg-1, indicating a complementary functional relationship. Therefore, natural symbiotic combinations exhibit various forms of symbiotic relationships, including complementarity, promotion, and inhibition, and there is a correlation between function and symbiotic relationship.

LIU Huiyao, LUO Tianxiong, WANG Ling, YANG Fusun. Investigation of the natural symbiotic relationship between mycorrhizal fungi and endophytic bacteria in Dendrobium nobile[J]. Journal of Tropical Biology, 2025, 16(2): 243-253. doi: 10.15886/j.cnki.rdswxb.20240098
Citation: LIU Huiyao, LUO Tianxiong, WANG Ling, YANG Fusun. Investigation of the natural symbiotic relationship between mycorrhizal fungi and endophytic bacteria in Dendrobium nobile[J]. Journal of Tropical Biology, 2025, 16(2): 243-253. doi: 10.15886/j.cnki.rdswxb.20240098
shu

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