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Volume 16 Issue 2
Apr.  2025
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Article Navigation >  Journal of Tropical Biology  > 2025  >  16(2): 181-187

HAN Mengmeng, HE Xiaotao, DUO Shuiyin, YANG Yuhui. Structural characterization of polysaccharide MSP-2 derived from Millettia speciosa Champ[J]. Journal of Tropical Biology, 2025, 16(2): 181-187. doi: 10.15886/j.cnki.rdswxb.20240066
Citation: HAN Mengmeng, HE Xiaotao, DUO Shuiyin, YANG Yuhui. Structural characterization of polysaccharide MSP-2 derived from Millettia speciosa Champ[J]. Journal of Tropical Biology, 2025, 16(2): 181-187. doi: 10.15886/j.cnki.rdswxb.20240066
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Structural characterization of polysaccharide MSP-2 derived from Millettia speciosa Champ

doi: 10.15886/j.cnki.rdswxb.20240066

  • School of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China

  • Received Date: 2024-04-15
  • Rev Recd Date: 2024-05-20
  • Polysaccharide（MSP-2） was extracted from Millettia speciosa Champ by hydroalcohol precipitation to analyze its chemical structure. The protein was removed by enzyme digestion and Sevag method, and then further purified by using anion-exchange column chromatography and gel filtration column chromatography to obtain MSP-2 fractions. The structure of the polysaccharide was analyzed in detail by using four analytical methods,including high performance anion exchange chromatography（HPAEC-PAD）, gel permeation（GPC-RIDMALLS）, Fourier infrared spectroscopy（FT-IR）, and nuclear magnetic resonance（ NMR）. The results showed that MSP-2 is a homogeneous neutral polysaccharide composed of glucopyranose with an average molecular weight（Mw） of 12.5 kDa and that its main backbone consists of T-α-D-Glcp,（1,4）-α-D-Glcp and（1,4,6）-α-DGlcp. Toxicity evaluation by MTT assay showed that MSP-2 was not toxic to RAW264.7 cells when its concentration was lower than 400 μg·mL-1.
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Structural characterization of polysaccharide MSP-2 derived from Millettia speciosa Champ

doi: 10.15886/j.cnki.rdswxb.20240066
  • School of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China
  • Received Date: 2024-04-15
  • Rev Recd Date: 2024-05-20

Abstract: Polysaccharide（MSP-2） was extracted from Millettia speciosa Champ by hydroalcohol precipitation to analyze its chemical structure. The protein was removed by enzyme digestion and Sevag method, and then further purified by using anion-exchange column chromatography and gel filtration column chromatography to obtain MSP-2 fractions. The structure of the polysaccharide was analyzed in detail by using four analytical methods,including high performance anion exchange chromatography（HPAEC-PAD）, gel permeation（GPC-RIDMALLS）, Fourier infrared spectroscopy（FT-IR）, and nuclear magnetic resonance（ NMR）. The results showed that MSP-2 is a homogeneous neutral polysaccharide composed of glucopyranose with an average molecular weight（Mw） of 12.5 kDa and that its main backbone consists of T-α-D-Glcp,（1,4）-α-D-Glcp and（1,4,6）-α-DGlcp. Toxicity evaluation by MTT assay showed that MSP-2 was not toxic to RAW264.7 cells when its concentration was lower than 400 μg·mL-1.

HAN Mengmeng, HE Xiaotao, DUO Shuiyin, YANG Yuhui. Structural characterization of polysaccharide MSP-2 derived from Millettia speciosa Champ[J]. Journal of Tropical Biology, 2025, 16(2): 181-187. doi: 10.15886/j.cnki.rdswxb.20240066
Citation: HAN Mengmeng, HE Xiaotao, DUO Shuiyin, YANG Yuhui. Structural characterization of polysaccharide MSP-2 derived from Millettia speciosa Champ[J]. Journal of Tropical Biology, 2025, 16(2): 181-187. doi: 10.15886/j.cnki.rdswxb.20240066
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