The Impact of <i>Eucalyptus</i> spp. dieback disease on soil bacterial diversity in forest stands

LIANG Chen; CHEN Zujing; YOU Guanghuai; WU Xiaohang; HUANG Huiming; HE Guankong; YANG Shengji

doi:10.15886/j.cnki.rdswxb.20240045

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Volume 16 Issue 2

Apr. 2025

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Article Navigation > Journal of Tropical Biology > 2025 > 16(2): 218-226

LIANG Chen, CHEN Zujing, YOU Guanghuai, WU Xiaohang, HUANG Huiming, HE Guankong, YANG Shengji. The Impact of Eucalyptus spp. dieback disease on soil bacterial diversity in forest stands[J]. Journal of Tropical Biology, 2025, 16(2): 218-226. doi: 10.15886/j.cnki.rdswxb.20240045

Citation:

LIANG Chen, CHEN Zujing, YOU Guanghuai, WU Xiaohang, HUANG Huiming, HE Guankong, YANG Shengji. The Impact of Eucalyptus spp. dieback disease on soil bacterial diversity in forest stands[J]. Journal of Tropical Biology, 2025, 16(2): 218-226. doi: 10.15886/j.cnki.rdswxb.20240045

The Impact of Eucalyptus spp. dieback disease on soil bacterial diversity in forest stands

doi: 10.15886/j.cnki.rdswxb.20240045

State-owned Bobai Forest Farm of Guangxi Zhuang Autonomous Region, Yulin, Guangxi 537600, China

Received Date: 2024-03-20
Rev Recd Date: 2024-05-25

Abstract

An attempt was made to explore the influence of Eucalyptus spp. dieback disease on soil bacteria under Eucalyptus spp. forests and its impact on the ecological mechanism of soil bacteria, providing a theoretical basis for revealing the pathogenesis of Eucalyptus spp. dieback disease and developing ecological prevention and control techniques for this disease. The soil bacterial DNA of Eucalyptus spp. forests affected and unaffected by dieback disease were sequenced using 16S rRNA. Data were statistically analyzed using SPSS 20.0, Microsoft Excel 2019, and R4.3.2 software, and images were processed using Adobe Illustrator 2021. A total of 2 537bacterial ASVs were detected in the soil of affected forests, and 2 477 ASVs were detected in unaffected soil.There was no significant difference in alpha diversity of bacteria between the two forest soils. The dominant phyla in both affected and unaffected（healthy） forest soils were Proteobacteria, Chloroflexi, and Actinobacteria, and the dominant genera were Acidothermus, norank＿f＿norank＿o＿Elsterales, and norank f＿Xanthobacteraceae.Significant differences in bacteria between the two forest soils were observed at the phylum level for Bacteroidota and Dependentiae, and at the genus level for norank＿f＿norank＿o＿Acidobacteriales, norank＿f＿norank＿o＿norank＿c＿AD3 and norank＿f＿norank＿o＿B12-WMSP1. There were more complex connections among microbial communities in the healthy forest soils compared to the affected ones. Eucalyptus spp. dieback disease does not significantly alter the diversity of soil bacteria under forests, and the soil still retains certain ecological regulation capabilities. However, differences between some bacterial communities suggest that Eucalyptus spp. dieback disease may potentially impact soil microbiota under Eucalyptus spp. forests.
- Eucalyptus spp.,
- Eucalyptus spp.dieback disease,
- soil bacteria,
- 16S rRNA,
- microbial diversity

References

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[2]	殷亚方,姜笑梅,吕建雄,等.我国桉树人工林资源和木材利用现状[J]. 木材工业, 2001, 15(5):3-5.
[3]	黄国勤,赵其国.广西桉树种植的历史、现状、生态问题及应对策略[J]. 生态学报, 2014, 34(18):5142-5152.
[4]	项东云,陈健波,叶露,等.广西桉树人工林发展现状、问题与对策[J]. 广西林业科学, 2006, 35(4):195-201.
[5]	冯淑芬,郑建华.海南岛小叶桉苗圃幼苗发生的一种新病害[J]. 热带作物研究, 1986, 6(1):59-61.
[6]	梁雪莹,林彦,卢万鸿. 14种桉树基因型对叶焦枯病病原菌抗病性研究[J]. 桉树科技, 2023, 40(3):1-10.
[7]	郑鹏.桉树焦枯病的发生特点及防治措施[J]. 广东蚕业, 2023, 57(5):24-26.
[8]	朱永官,沈仁芳,贺纪正,等.中国土壤微生物组:进展与展望[J]. 中国科学院院刊, 2017, 32(6):554-565.
[9]	张晶,张惠文,李新宇,等.土壤微生物生态过程与微生物功能基因多样性[J]. 应用生态学报, 2006, 17(6):1129-1132.
[10]	曹志平.土壤生态学[M]. 北京:化学工业出版社,2007:211-222.
[11]	TRIVEDI P, DELGADO-BAQUERIZO M, TRIVEDI C,et al. Keystone microbial taxa regulate the invasion of a fungal pathogen in agro-ecosystems[J]. Soil Biology and Biochemistry, 2017, 111:10-14.
[12]	魏兰芳,张荣琴,姚博,等.大豆轮作及秸秆还田模式对白菜根肿病的影响[J]. 江西农业大学学报, 2021,43(1):52-62.
[13]	陈巧环,苗玉焕,王铁霖,等.枯萎病对菊花根际土壤微生物群落结构的影响[J]. 中国实验方剂学杂志,2021, 27(11):180-186.
[14]	伍文宪,黄小琴,张蕾,等.十字花科作物根肿病对根际土壤微生物群落的影响[J]. 生态学报, 2020, 40(5):1532-1541.
[15]	史普酉,杨成翠,贾孟,等.不同黑胫病发病程度下植烟根际土壤酶活性及细菌群落结构差异比较[J]. 中国土壤与肥料, 2020(1):179-187.
[16]	任利利,王焱,马风林,等.上海地区果树根癌病发生与土壤环境的关系[J]. 生态环境, 2007, 16(2):498-502.
[17]	杨婕.桉树抗焦枯病EuPOD和EuPAL基因的克隆与功能分析[D]. 福州:福建农林大学, 2016.
[18]	汪全超.雷州半岛桉树叶焦枯病病原菌鉴定及不同桉树基因型抗病性研究[D]. 北京:中国林业科学研究院, 2020.
[19]	孝惠爽,赵杰,傅声雷.华南典型尾叶桉纯林经营对土壤理化性质、微生物和线虫群落的影响[J]. 生态学报, 2023, 43(19):7963-7973.
[20]	BULGARELLI R G, LEITE M F A, DE HOLLANDER M, et al. Eucalypt species drive rhizosphere bacterial and fungal community assembly but soil phosphorus availability rearranges the microbiome[J]. The Science of the Total Environment, 2022, 836:155667.
[21]	QIN F, YANG F, MING A, et al. Mixture enhances microbial network complexity of soil carbon, nitrogen and phosphorus cycling in Eucalyptus plantations[J]. Forest Ecology and Management, 2024, 553:121632.
[22]	马瑗蕊,石艳平,黄其颖,等.水肥和钝化剂阻控水稻吸收镉机制的研究进展[J]. 中国稻米, 2024, 30(1):10-17.
[23]	KAGEYAMA K, ASANO T. Life cycle of Plasmodiophora brassicae[J]. Journal of Plant Growth Regulation,2009, 28(3):203-211.
[24]	赵子麒.尾巨桉人工林土壤细菌群落结构和多样性的动态变化[D]. 长沙:中南林业科技大学, 2021.
[25]	许宇星,王志超,竹万宽,等.桉树多代连栽引起的土壤细菌群落结构及共现性网络特征变化[J]. 桉树科技, 2022, 39(3):1-8.
[26]	徐海忠,薛彦华,丁洪发,等.不同地区桃树根际土壤微生物群落结构及多样性分析[J]. 中国果菜, 2024,44(1):72-79.
[27]	欧光敏,郑良豹,梁红,等.猕猴桃林下套种大豆土壤微生物群落结构分析[J]. 广东农业科学, 2024, 51(1):63-72.
[28]
[29]	雷美玲,饶文华,胡进锋,等.黄龙病发病芦柑根际土壤细菌群落组成与多样性特征[J]. 生物技术通报,2024, 40(2):266-276.
[30]	彭新红,陈绪涛,何虎,等.大球盖菇菌渣还田对水稻生长和稻田土壤环境的影响[J]. 江西农业大学学报,2023, 45(2):349-360.
[31]	吕嘉妍,毛健辉,霍春宇,等.广东省本地油茶和引种油茶根际土壤微生物群落特征[J]. 微生物学通报,2023, 50(11):4938-4953.
[32]	汪运祥,吴航湦,何冬梅,等.郭岩山自然保护区丝栗栲天然林分土壤微生物群落沿海拔梯度变化[J]. 林业科学研究, 2023, 36(6):134-143.
[33]	CHAPARRO J M, SHEFLIN A M, MANTER D K, et al.Manipulating the soil microbiome to increase soil health and plant fertility[J]. Biology and Fertility of Soils, 2012,48(5):489-499.
[34]	范适,杨宁.亚热带5个生态系统土壤微生物学性质比较[J]. 热带亚热带植物学报, 2016, 24(6):635-641.
[35]	XIONG W, ZHAO Q, ZHAO J, et al. Different continuous cropping spans significantly affect microbial community membership and structure in a Vanilla-grown soil as revealed by deep pyrosequencing[J]. Microbial Ecology,2015, 70(1):209-218.
[36]	JONES R T, ROBESON M S, LAUBER C L, et al. A comprehensive survey of soil acidobacterial diversity using pyrosequencing and clone library analyses[J]. The ISME Journal, 2009, 3(4):442-453.
[37]	倪苗,成善汉,韩旭,等.轮作不同叶菜对连作豇豆土壤养分及微生物特性的影响[J]. 中国蔬菜, 2019(5):64-69.
[38]	谢仕祺.土壤调理剂对凉山州植烟土壤微生物区系的影响及其相关性分析[D]. 郑州:郑州大学, 2021.
[39]	SCHULZ-BOHM K, GERARDS S, HUNDSCHEID M, et al. Calling from distance:attraction of soil bacteria by plant root volatiles[J]. The ISME Journal, 2018, 12:1252-1262.
[40]	KINKEL L L, BAKKER M G, SCHLATTER D C. A coevolutionary framework for managing diseasesuppressive soils[J]. Annual Review of Phytopathology,2011, 49:47-67.
[41]	李善家,王辉,苟伟,等.混生荒漠植物叶片功能性状与其根际微生物多样性的关系[J]. 生态环境学报,2020, 29(9):1713-1722.
[42]	CHEN S F, LOMBARD L, ROUX J, et al. Novel species of Calonectria associated with Eucalyptus leaf blight in Southeast China[J]. Persoonia, 2011, 26:1-12.
[43]	LOMBARD L, CHEN S F, MOU X, et al. New species,hyper-diversity and potential importance of Calonectria spp. from Eucalyptus in South China[J]. Studies in Mycology, 2015, 80:151-188.
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The Impact of Eucalyptus spp. dieback disease on soil bacterial diversity in forest stands

State-owned Bobai Forest Farm of Guangxi Zhuang Autonomous Region, Yulin, Guangxi 537600, China

Received Date: 2024-03-20

Rev Recd Date: 2024-05-25

Key words:

Abstract: An attempt was made to explore the influence of Eucalyptus spp. dieback disease on soil bacteria under Eucalyptus spp. forests and its impact on the ecological mechanism of soil bacteria, providing a theoretical basis for revealing the pathogenesis of Eucalyptus spp. dieback disease and developing ecological prevention and control techniques for this disease. The soil bacterial DNA of Eucalyptus spp. forests affected and unaffected by dieback disease were sequenced using 16S rRNA. Data were statistically analyzed using SPSS 20.0, Microsoft Excel 2019, and R4.3.2 software, and images were processed using Adobe Illustrator 2021. A total of 2 537bacterial ASVs were detected in the soil of affected forests, and 2 477 ASVs were detected in unaffected soil.There was no significant difference in alpha diversity of bacteria between the two forest soils. The dominant phyla in both affected and unaffected（healthy） forest soils were Proteobacteria, Chloroflexi, and Actinobacteria, and the dominant genera were Acidothermus, norank＿f＿norank＿o＿Elsterales, and norank f＿Xanthobacteraceae.Significant differences in bacteria between the two forest soils were observed at the phylum level for Bacteroidota and Dependentiae, and at the genus level for norank＿f＿norank＿o＿Acidobacteriales, norank＿f＿norank＿o＿norank＿c＿AD3 and norank＿f＿norank＿o＿B12-WMSP1. There were more complex connections among microbial communities in the healthy forest soils compared to the affected ones. Eucalyptus spp. dieback disease does not significantly alter the diversity of soil bacteria under forests, and the soil still retains certain ecological regulation capabilities. However, differences between some bacterial communities suggest that Eucalyptus spp. dieback disease may potentially impact soil microbiota under Eucalyptus spp. forests.

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Reference (44)

[1]	王震洪,段昌群,起联春,等.我国桉树林发展中的生态问题探讨[J]. 生态学杂志, 1998, 17(6):65-69.
[2]	殷亚方,姜笑梅,吕建雄,等.我国桉树人工林资源和木材利用现状[J]. 木材工业, 2001, 15(5):3-5.
[3]	黄国勤,赵其国.广西桉树种植的历史、现状、生态问题及应对策略[J]. 生态学报, 2014, 34(18):5142-5152.
[4]	项东云,陈健波,叶露,等.广西桉树人工林发展现状、问题与对策[J]. 广西林业科学, 2006, 35(4):195-201.
[5]	冯淑芬,郑建华.海南岛小叶桉苗圃幼苗发生的一种新病害[J]. 热带作物研究, 1986, 6(1):59-61.
[6]	梁雪莹,林彦,卢万鸿. 14种桉树基因型对叶焦枯病病原菌抗病性研究[J]. 桉树科技, 2023, 40(3):1-10.
[7]	郑鹏.桉树焦枯病的发生特点及防治措施[J]. 广东蚕业, 2023, 57(5):24-26.
[8]	朱永官,沈仁芳,贺纪正,等.中国土壤微生物组:进展与展望[J]. 中国科学院院刊, 2017, 32(6):554-565.
[9]	张晶,张惠文,李新宇,等.土壤微生物生态过程与微生物功能基因多样性[J]. 应用生态学报, 2006, 17(6):1129-1132.
[10]	曹志平.土壤生态学[M]. 北京:化学工业出版社,2007:211-222.
[11]	TRIVEDI P, DELGADO-BAQUERIZO M, TRIVEDI C,et al. Keystone microbial taxa regulate the invasion of a fungal pathogen in agro-ecosystems[J]. Soil Biology and Biochemistry, 2017, 111:10-14.
[12]	魏兰芳,张荣琴,姚博,等.大豆轮作及秸秆还田模式对白菜根肿病的影响[J]. 江西农业大学学报, 2021,43(1):52-62.
[13]	陈巧环,苗玉焕,王铁霖,等.枯萎病对菊花根际土壤微生物群落结构的影响[J]. 中国实验方剂学杂志,2021, 27(11):180-186.
[14]	伍文宪,黄小琴,张蕾,等.十字花科作物根肿病对根际土壤微生物群落的影响[J]. 生态学报, 2020, 40(5):1532-1541.
[15]	史普酉,杨成翠,贾孟,等.不同黑胫病发病程度下植烟根际土壤酶活性及细菌群落结构差异比较[J]. 中国土壤与肥料, 2020(1):179-187.
[16]	任利利,王焱,马风林,等.上海地区果树根癌病发生与土壤环境的关系[J]. 生态环境, 2007, 16(2):498-502.
[17]	杨婕.桉树抗焦枯病EuPOD和EuPAL基因的克隆与功能分析[D]. 福州:福建农林大学, 2016.
[18]	汪全超.雷州半岛桉树叶焦枯病病原菌鉴定及不同桉树基因型抗病性研究[D]. 北京:中国林业科学研究院, 2020.
[19]	孝惠爽,赵杰,傅声雷.华南典型尾叶桉纯林经营对土壤理化性质、微生物和线虫群落的影响[J]. 生态学报, 2023, 43(19):7963-7973.
[20]	BULGARELLI R G, LEITE M F A, DE HOLLANDER M, et al. Eucalypt species drive rhizosphere bacterial and fungal community assembly but soil phosphorus availability rearranges the microbiome[J]. The Science of the Total Environment, 2022, 836:155667.
[21]	QIN F, YANG F, MING A, et al. Mixture enhances microbial network complexity of soil carbon, nitrogen and phosphorus cycling in Eucalyptus plantations[J]. Forest Ecology and Management, 2024, 553:121632.
[22]	马瑗蕊,石艳平,黄其颖,等.水肥和钝化剂阻控水稻吸收镉机制的研究进展[J]. 中国稻米, 2024, 30(1):10-17.
[23]	KAGEYAMA K, ASANO T. Life cycle of Plasmodiophora brassicae[J]. Journal of Plant Growth Regulation,2009, 28(3):203-211.
[24]	赵子麒.尾巨桉人工林土壤细菌群落结构和多样性的动态变化[D]. 长沙:中南林业科技大学, 2021.
[25]	许宇星,王志超,竹万宽,等.桉树多代连栽引起的土壤细菌群落结构及共现性网络特征变化[J]. 桉树科技, 2022, 39(3):1-8.
[26]	徐海忠,薛彦华,丁洪发,等.不同地区桃树根际土壤微生物群落结构及多样性分析[J]. 中国果菜, 2024,44(1):72-79.
[27]	欧光敏,郑良豹,梁红,等.猕猴桃林下套种大豆土壤微生物群落结构分析[J]. 广东农业科学, 2024, 51(1):63-72.
[28]
[29]	雷美玲,饶文华,胡进锋,等.黄龙病发病芦柑根际土壤细菌群落组成与多样性特征[J]. 生物技术通报,2024, 40(2):266-276.
[30]	彭新红,陈绪涛,何虎,等.大球盖菇菌渣还田对水稻生长和稻田土壤环境的影响[J]. 江西农业大学学报,2023, 45(2):349-360.
[31]	吕嘉妍,毛健辉,霍春宇,等.广东省本地油茶和引种油茶根际土壤微生物群落特征[J]. 微生物学通报,2023, 50(11):4938-4953.
[32]	汪运祥,吴航湦,何冬梅,等.郭岩山自然保护区丝栗栲天然林分土壤微生物群落沿海拔梯度变化[J]. 林业科学研究, 2023, 36(6):134-143.
[33]	CHAPARRO J M, SHEFLIN A M, MANTER D K, et al.Manipulating the soil microbiome to increase soil health and plant fertility[J]. Biology and Fertility of Soils, 2012,48(5):489-499.
[34]	范适,杨宁.亚热带5个生态系统土壤微生物学性质比较[J]. 热带亚热带植物学报, 2016, 24(6):635-641.
[35]	XIONG W, ZHAO Q, ZHAO J, et al. Different continuous cropping spans significantly affect microbial community membership and structure in a Vanilla-grown soil as revealed by deep pyrosequencing[J]. Microbial Ecology,2015, 70(1):209-218.
[36]	JONES R T, ROBESON M S, LAUBER C L, et al. A comprehensive survey of soil acidobacterial diversity using pyrosequencing and clone library analyses[J]. The ISME Journal, 2009, 3(4):442-453.
[37]	倪苗,成善汉,韩旭,等.轮作不同叶菜对连作豇豆土壤养分及微生物特性的影响[J]. 中国蔬菜, 2019(5):64-69.
[38]	谢仕祺.土壤调理剂对凉山州植烟土壤微生物区系的影响及其相关性分析[D]. 郑州:郑州大学, 2021.
[39]	SCHULZ-BOHM K, GERARDS S, HUNDSCHEID M, et al. Calling from distance:attraction of soil bacteria by plant root volatiles[J]. The ISME Journal, 2018, 12:1252-1262.
[40]	KINKEL L L, BAKKER M G, SCHLATTER D C. A coevolutionary framework for managing diseasesuppressive soils[J]. Annual Review of Phytopathology,2011, 49:47-67.
[41]	李善家,王辉,苟伟,等.混生荒漠植物叶片功能性状与其根际微生物多样性的关系[J]. 生态环境学报,2020, 29(9):1713-1722.
[42]	CHEN S F, LOMBARD L, ROUX J, et al. Novel species of Calonectria associated with Eucalyptus leaf blight in Southeast China[J]. Persoonia, 2011, 26:1-12.
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The Impact of Eucalyptus spp. dieback disease on soil bacterial diversity in forest stands

doi: 10.15886/j.cnki.rdswxb.20240045

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The Impact of Eucalyptus spp. dieback disease on soil bacterial diversity in forest stands

doi: 10.15886/j.cnki.rdswxb.20240045

State-owned Bobai Forest Farm of Guangxi Zhuang Autonomous Region, Yulin, Guangxi 537600, China

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The Impact of Eucalyptus spp. dieback disease on soil bacterial diversity in forest stands

doi: 10.15886/j.cnki.rdswxb.20240045

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通讯作者: 陈斌, bchen63@163.com

Proportional views

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The Impact of Eucalyptus spp. dieback disease on soil bacterial diversity in forest stands

doi: 10.15886/j.cnki.rdswxb.20240045

State-owned Bobai Forest Farm of Guangxi Zhuang Autonomous Region, Yulin, Guangxi 537600, China

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