Methylcyclohexanone metabolites from endophytic Aspergillus sp. HU0206 have inhibitory activity against Xanthomonas oryzae pv. oryzae

REN Qinqin; CHEN Shaowei; ZHENG Jiahua; WANG Rui; XU Liangxiong; TAO Jun

doi:10.15886/j.cnki.rdswxb.20240010

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During the continuing investigation of the antibacterial metabolites from endophytic Aspergillus sp. HU0206,six compounds were isolated from the fermented culture of this fungus by column chromatography and high-performance liquid preparation. These compounds were elucidated as four methylcyclohexanones: ampelomin A（ 1 ）, 7-hydroxylampelomin A（ 2 ）, 7-O-ethylampelomin A（ 3 ）, 2,3-dihydroxyl-7-O-ethylampelomin A（ 4 ） and two methylphenols:2-（methoxymethyl）-1,4-benzenediol（ 5 ） and 3-chlorogentisyl alcohol（ 6 ） on the basis of NMR and ESI-MS analyses.Compounds 2-4 are new compounds. At the dose of 40 μg, compounds 1, 2 and 6 showed obvious antibacterial effects on Xanthomonas oryzae with inhibition zones of 4.0 mm. Compound 6 also exhibited inhibitory activities against Staphylococcus aureus, methicillin-resistant S. aureus, Pectobacterium atroseptica and Ralstonia solanacearum. However,compounds 3-5 showed no inhibitory activity against X. oryzae and compounds 1-5 were inactive against the other four strains mentioned above. The biosynthesis pathway of the metabolites from Aspergillus sp. HU0206 was also discussed.

Methylcyclohexanone metabolites from endophytic Aspergillus sp. HU0206 have inhibitory activity against Xanthomonas oryzae pv. oryzae

1. Key Laboratory of Sustainable Utilization of Tropical Biological Resources of Hainan Province/School of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, china;

2. School of Life Sciences, Huizhou University, Huizhou, Guangdong 516007, China

Received Date: 2024-01-18

Rev Recd Date: 2024-05-18

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Abstract: During the continuing investigation of the antibacterial metabolites from endophytic Aspergillus sp. HU0206,six compounds were isolated from the fermented culture of this fungus by column chromatography and high-performance liquid preparation. These compounds were elucidated as four methylcyclohexanones: ampelomin A（ 1 ）, 7-hydroxylampelomin A（ 2 ）, 7-O-ethylampelomin A（ 3 ）, 2,3-dihydroxyl-7-O-ethylampelomin A（ 4 ） and two methylphenols:2-（methoxymethyl）-1,4-benzenediol（ 5 ） and 3-chlorogentisyl alcohol（ 6 ） on the basis of NMR and ESI-MS analyses.Compounds 2-4 are new compounds. At the dose of 40 μg, compounds 1, 2 and 6 showed obvious antibacterial effects on Xanthomonas oryzae with inhibition zones of 4.0 mm. Compound 6 also exhibited inhibitory activities against Staphylococcus aureus, methicillin-resistant S. aureus, Pectobacterium atroseptica and Ralstonia solanacearum. However,compounds 3-5 showed no inhibitory activity against X. oryzae and compounds 1-5 were inactive against the other four strains mentioned above. The biosynthesis pathway of the metabolites from Aspergillus sp. HU0206 was also discussed.

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Methylcyclohexanone metabolites from endophytic Aspergillus sp. HU0206 have inhibitory activity against Xanthomonas oryzae pv. oryzae

doi: 10.15886/j.cnki.rdswxb.20240010

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