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Volume 6 Issue 3
Sep.  2015
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Article Navigation >  Journal of Tropical Biology  > 2015  >  6(3): 325-328

ZHANG Yuwen, XIE Xueli, HUANG Mianjia, LI Jiatong, WU Lanfang. Comparison of Vacuum Concentration and Rotary Evaporation Methods in Artemisinin Extraction[J]. Journal of Tropical Biology, 2015, 6(3): 325-328. doi: 10.15886/j.cnki.rdswxb.2015.03.018
Citation: ZHANG Yuwen, XIE Xueli, HUANG Mianjia, LI Jiatong, WU Lanfang. Comparison of Vacuum Concentration and Rotary Evaporation Methods in Artemisinin Extraction[J]. Journal of Tropical Biology, 2015, 6(3): 325-328. doi: 10.15886/j.cnki.rdswxb.2015.03.018
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Comparison of Vacuum Concentration and Rotary Evaporation Methods in Artemisinin Extraction

doi: 10.15886/j.cnki.rdswxb.2015.03.018

  • 1. College of Horticulture and Landscape Architecture, Hainan University, Haikou 570228;

  • 2. Hainan Research Center for Drug Safety Evaluation, Hainan Medical University, Haikou 571199, China

  • Received Date: 2015-02-10
  • Artemisinin was extracted from mature leaves of Artemisia annua L. by using a rotary evaporation method or a vacuum concentration method to establish an effective,simple method for artemisinin extraction.The absorbance of artemisinin was determined at the wavelength of 292 nm by using ultraviolet spectrophotometer. The rotary evaporation method extracted artemisinin at an average recovery rate of 92. 18% with the RSD being 0. 91%,and the vacuum concentration method extracted artemisinin at an average recovery rate of 92. 90%with the RSD being 0. 77%. These two methods had respective RSDs of 1. 11% and 1. 61% in the precision test,1. 53% and 1. 70% in a stability test,and 0. 76% and 1. 03% in the repeatability test. It is concluded that the vacuum concentration method was simpler and more rapid and feasible than the rotary evaporation method for extraction of artemisinin from the leaves of A. annua L.
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Comparison of Vacuum Concentration and Rotary Evaporation Methods in Artemisinin Extraction

doi: 10.15886/j.cnki.rdswxb.2015.03.018
  • 1. College of Horticulture and Landscape Architecture, Hainan University, Haikou 570228;
  • 2. Hainan Research Center for Drug Safety Evaluation, Hainan Medical University, Haikou 571199, China
  • Received Date: 2015-02-10

Abstract: Artemisinin was extracted from mature leaves of Artemisia annua L. by using a rotary evaporation method or a vacuum concentration method to establish an effective,simple method for artemisinin extraction.The absorbance of artemisinin was determined at the wavelength of 292 nm by using ultraviolet spectrophotometer. The rotary evaporation method extracted artemisinin at an average recovery rate of 92. 18% with the RSD being 0. 91%,and the vacuum concentration method extracted artemisinin at an average recovery rate of 92. 90%with the RSD being 0. 77%. These two methods had respective RSDs of 1. 11% and 1. 61% in the precision test,1. 53% and 1. 70% in a stability test,and 0. 76% and 1. 03% in the repeatability test. It is concluded that the vacuum concentration method was simpler and more rapid and feasible than the rotary evaporation method for extraction of artemisinin from the leaves of A. annua L.

ZHANG Yuwen, XIE Xueli, HUANG Mianjia, LI Jiatong, WU Lanfang. Comparison of Vacuum Concentration and Rotary Evaporation Methods in Artemisinin Extraction[J]. Journal of Tropical Biology, 2015, 6(3): 325-328. doi: 10.15886/j.cnki.rdswxb.2015.03.018
Citation: ZHANG Yuwen, XIE Xueli, HUANG Mianjia, LI Jiatong, WU Lanfang. Comparison of Vacuum Concentration and Rotary Evaporation Methods in Artemisinin Extraction[J]. Journal of Tropical Biology, 2015, 6(3): 325-328. doi: 10.15886/j.cnki.rdswxb.2015.03.018
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