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Volume 6 Issue 3
Sep.  2015
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Article Navigation >  Journal of Tropical Biology  > 2015  >  6(3): 261-268

ZHANG Qiaoling, RONG Wei, LI Huiping, LIN Daozhe, HE Chaozu. The Functional Study of a Type Ⅲ Effector Protein XopR in Xanthomonas campestris pv. campestris[J]. Journal of Tropical Biology, 2015, 6(3): 261-268. doi: 10.15886/j.cnki.rdswxb.2015.03.007
Citation: ZHANG Qiaoling, RONG Wei, LI Huiping, LIN Daozhe, HE Chaozu. The Functional Study of a Type Ⅲ Effector Protein XopR in Xanthomonas campestris pv. campestris[J]. Journal of Tropical Biology, 2015, 6(3): 261-268. doi: 10.15886/j.cnki.rdswxb.2015.03.007
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The Functional Study of a Type Ⅲ Effector Protein XopR in Xanthomonas campestris pv. campestris

doi: 10.15886/j.cnki.rdswxb.2015.03.007

  • Hainan Key Laboratory of Sustainable Utilization of Tropical bioresources, Hainan University, Haikou 570228, China

  • Received Date: 2015-01-30
  • Xanthomonas campestris pathovar campestris（Xcc） is the causal agent of black rot disease on cruciferous plants. Xcc deliver many effector proteins into host plant cells via the type Ⅲ secretion system to help its infection and multiplication. In this study,the expression and regulation of XopR gene were analyzed through qRTPCR. The results showed that the XopR gene transcription was regulated by the type Ⅲ regulator of HrpG and HrpX,and the XopR protein secretion was type Ⅲ secretion system dependent,indicating that XopR was a typeⅢ effector protein ofXcc8004. In addition,a XopR gene deletion mutant（ Xcc8004,ΔXopR） was generated,and the disease symptoms caused by ΔXopR strain were decreased on Zhonggan15,Zhonggan21,Zhongbai83 in contrast with WTXcc8004,suggesting that XopR was required for the full virulence ofXcc8004 on Cabbage host plants. Furthermore,XopR was proved to located in plant plasma membrane in Arabidopsis protoplasts cells by XopR-GFP fusion assay. The expression of PTI marker gene FRK1∷LUC was inhibited by XopR protein,which was consistent with the XopR function in Xoo strains.
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The Functional Study of a Type Ⅲ Effector Protein XopR in Xanthomonas campestris pv. campestris

doi: 10.15886/j.cnki.rdswxb.2015.03.007
  • Hainan Key Laboratory of Sustainable Utilization of Tropical bioresources, Hainan University, Haikou 570228, China
  • Received Date: 2015-01-30

Abstract: Xanthomonas campestris pathovar campestris（Xcc） is the causal agent of black rot disease on cruciferous plants. Xcc deliver many effector proteins into host plant cells via the type Ⅲ secretion system to help its infection and multiplication. In this study,the expression and regulation of XopR gene were analyzed through qRTPCR. The results showed that the XopR gene transcription was regulated by the type Ⅲ regulator of HrpG and HrpX,and the XopR protein secretion was type Ⅲ secretion system dependent,indicating that XopR was a typeⅢ effector protein ofXcc8004. In addition,a XopR gene deletion mutant（ Xcc8004,ΔXopR） was generated,and the disease symptoms caused by ΔXopR strain were decreased on Zhonggan15,Zhonggan21,Zhongbai83 in contrast with WTXcc8004,suggesting that XopR was required for the full virulence ofXcc8004 on Cabbage host plants. Furthermore,XopR was proved to located in plant plasma membrane in Arabidopsis protoplasts cells by XopR-GFP fusion assay. The expression of PTI marker gene FRK1∷LUC was inhibited by XopR protein,which was consistent with the XopR function in Xoo strains.

ZHANG Qiaoling, RONG Wei, LI Huiping, LIN Daozhe, HE Chaozu. The Functional Study of a Type Ⅲ Effector Protein XopR in Xanthomonas campestris pv. campestris[J]. Journal of Tropical Biology, 2015, 6(3): 261-268. doi: 10.15886/j.cnki.rdswxb.2015.03.007
Citation: ZHANG Qiaoling, RONG Wei, LI Huiping, LIN Daozhe, HE Chaozu. The Functional Study of a Type Ⅲ Effector Protein XopR in Xanthomonas campestris pv. campestris[J]. Journal of Tropical Biology, 2015, 6(3): 261-268. doi: 10.15886/j.cnki.rdswxb.2015.03.007
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