[1] Nolan T, Hands R E, Bustin S A. Quantification of mRNA using real-time RT-PCR [J]. Nature Protocols, 2006, 1(3): 1559−1582. https://doi.org/10.1038/nprot.2006.236 doi:  10.1038/nprot.2006.236
[2] Sowa S, Sozoniuk M, Toporowska J, et al. Validation of reference genes as an internal control for studying Avena sativa–Puccinia coronata interaction by RT-qPCR [J]. Scientific Reports, 2022, 12(1): 14601. https://doi.org/10.1038/s41598-022-18746-z doi:  10.1038/s41598-022-18746-z
[3] Su B, Li Z, Liu H, et al. Identification and validation of reference genes for RT-qPCR analysis in Iris domestica under Cd stress [J]. Heliyon, 2024, 10(17): e36923. https://doi.org/10.1016/j.heliyon.2024.e36923 doi:  10.1016/j.heliyon.2024.e36923
[4] Yang Y, Cao G, Tang L. Selection and validation of reference genes for qRT-PCR normalization in dayflower (Commelina communis) based on the transcriptome profiling [J]. BMC Plant Biology, 2024, 24(1): 1131−1143. https://doi.org/10.1186/s12870-024-05853-4 doi:  10.1186/s12870-024-05853-4
[5] Vanguilder H D, Vrana K E, Freeman W M. Twenty-five years of quantitative PCR for gene expression analysis [J]. BioTechniques, 2008, 44(5): 619−626. https://doi.org/10.2144/000112776 doi:  10.2144/000112776
[6] Tang X, Zhang N, Si H, et al. Selection and validation of reference genes for RT-qPCR analysis in potato under abiotic stress [J]. Plant Methods, 2017, 13(1): 85. https://doi.org/10.1186/s13007-017-0238-7 doi:  10.1186/s13007-017-0238-7
[7] Zhang J, He X, Zhou J, et al. Selection and verification of standardized reference genes of Angelica dahurica under various abiotic stresses by real-time quantitative PCR [J]. Genes, 2024, 15(1): 79−95. https://doi.org/10.3390/genes15010079 doi:  10.3390/genes15010079
[8] Jeong Y M, Mun J H, Lee I, et al. Distinct roles of the first introns on the expression of Arabidopsis profilin gene family members [J]. Plant Physiology, 2006, 140(1): 196−209. https://doi.org/10.1104/pp.105.071316 doi:  10.1104/pp.105.071316
[9] 方佳, 濮文辉, 张慧坚. 国内外木薯产业发展近况[J]. 中国农学通报, 2010, 26(16): 353−361.
[10] 梁露锋, 玉琼广, 刘洁. 木薯产业发展动态及展望前景[J]. 大众科技, 2011, 128(6): 128−130. https://doi.org/10.3969/j.issn.1008-1151.2011.06.057 doi:  10.3969/j.issn.1008-1151.2011.06.057
[11] 严华兵, 叶剑秋, 李开绵. 中国木薯育种研究进展[J]. 中国农学通报, 2015, 31(15): 63−70. https://doi.org/10.11924/j.issn.1000-6850.casb14110159 doi:  10.11924/j.issn.1000-6850.casb14110159
[12] 刘佳, 张箭. 美洲木薯在非洲的引种和推广[J]. 世界农业, 2019, 14(2): 83−88.
[13] 陈松笔, 蔡杰, 安飞飞, 等. 木薯育种现状及发展趋势[J]. 中国科学: 生命科学, 2024, 54(10): 1833−1842.
[14]

Amelework A B, Bairu M W. Advances in genetic analysis and breeding of cassava (Manihot esculenta Crantz): A review [J]. Plants, 2022, 11(12): 1617−1635. https://doi.org/10.3390/plants11121617 doi:  10.3390/plants11121617
[15]

Carlos A Z -C, Cruz G D, Verdier V, et al. Cassava diseases caused by Xanthomonas phaseoli pv. manihotis cassavae [J]. Molecular Plant Pathology, 2021, 22(12): 1520−1537. https://doi.org/10.1111/mpp.13094 doi:  10.1111/mpp.13094
[16] 李超萍, 时涛, 刘先宝, 等. 国内木薯病害普查及细菌性萎蔫病安全性评估[J]. 热带作物学报, 2011, 32(1): 116−121.
[17]

Carlosa Z C, Moufid Y, López C E, et al. First report of cassava bacterial blight caused by Xanthomonas phaseoli pv. manihotis in the amazonian forest of ecuador [J]. Plant Disease, 2024, 108(6): 1879.
[18]

Hu M, Hu W, Xia Z, et al. Validation of reference genes for relative quantitative gene expression studies in cassava (Manihot esculenta Crantz) by using quantitative real-time PCR [J]. Frontiers in Plant Science, 2016, 7: 680.
[19]

Moreno I, Gruissem W, Vanderschuren H. Reference genes for reliable potyvirus quantitation in cassava and analysis of Cassava brown streak virus load in host varieties [J]. Journal of Virological Methods, 2011, 177(1): 49−54. https://doi.org/10.1016/j.jviromet.2011.06.013 doi:  10.1016/j.jviromet.2011.06.013
[20]

Czechowski T, Stitt M, Altmann T, et al. Genome-wide identification and testing of superior reference genes for transcript normalization in Arabidopsis [J]. Plant Physiology, 2005, 139(1): 5−17. https://doi.org/10.1104/pp.105.063743 doi:  10.1104/pp.105.063743
[21]

Chang Y, Bai Y, Wei Y, et al. CAMTA3 negatively regulates disease resistance through modulating immune response and extensive transcriptional reprogramming in cassava [J]. Tree Physiology, 2020, 40(11): 1520−1533. https://doi.org/10.1093/treephys/tpaa093 doi:  10.1093/treephys/tpaa093
[22]

Zheng L, Gao S, Bai Y, et al. NF‐YC15 transcription factor activates ethylene biosynthesis and improves cassava disease resistance [J]. Plant Biotechnology Journal, 2024, 22(9): 2424−2434. https://doi.org/10.1111/pbi.14355 doi:  10.1111/pbi.14355
[23]

Zhu S, Pan Y, Li K, et al. Complete genome sequence of Xanthomonas phaseoli pv. manihotis strain CHN01, the causal agent of cassava bacterial blight [J]. Plant Disease, 2022, 106(3): 1039−1041. https://doi.org/10.1094/PDIS-09-21-2016-A doi:  10.1094/PDIS-09-21-2016-A
[24]

Wei Y, Shi H, Xia Z, et al. Genome-wide identification and expression analysis of the WRKY gene family in cassava [J]. Frontiers in Plant Science, 2016, 7: 25.
[25]

Tong Z, Gao Z, Wang F, et al. Selection of reliable reference genes for gene expression studies in peach using real-time PCR [J]. BMC Molecular Biology, 2009, 10: 71. https://doi.org/10.1186/1471-2199-10-71 doi:  10.1186/1471-2199-10-71
[26]

Zhong H Y, Chen J W, Li C Q, et al. Selection of reliable reference genes for expression studies by reverse transcription quantitative real-time PCR in litchi under different experimental conditions [J]. Plant Cell Reports, 2011, 30(4): 641−653. https://doi.org/10.1007/s00299-010-0992-8 doi:  10.1007/s00299-010-0992-8
[27]

Nicot N, Hausman J F, Hoffmann L, et al. Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress [J]. Journal of Experimental Botany, 2005, 56(421): 2907−2914. https://doi.org/10.1093/jxb/eri285 doi:  10.1093/jxb/eri285
[28]

Gao K, Khan Wu, LI J, et al. Identification and validation of reliable reference genes for gene expression studies in Koelreuteria paniculata [J]. Genes, 2022, 13(5): 714−725. https://doi.org/10.3390/genes13050714 doi:  10.3390/genes13050714
[29]

Li X, Cheng J, Zhang J, et al. Validation of reference genes for accurate normalization of gene expression in Lilium davidii var. unicolor for real time quantitative PCR [J]. Plos One, 2015, 10(10): 28−43.
[30]

Li G, Sun X, Zhu X, et al. Selection and validation of reference genes in virus-infected sweet potato plants [J]. Genes, 2023, 14(7): 1477−1488. https://doi.org/10.3390/genes14071477 doi:  10.3390/genes14071477
[31]

Mascia T, Santovito E, Gallitelli D, et al. Evaluation of reference genes for quantitative reverse‐transcription polymerase chain reaction normalization in infected tomato plants [J]. Molecular Plant Pathology, 2010, 11(6): 805−816. https://doi.org/10.1111/j.1364-3703.2010.00646.x doi:  10.1111/j.1364-3703.2010.00646.x
[32] 唐枝娟, 刘秦, 肖晓蓉, 等. 白叶枯病菌侵染下的水稻内参基因稳定性[J]. 分子植物育种, 2017, 15(1): 300−306.