[1] KITAGAWA H, OHGE H, YU L, et al. Aeromonas dhakensis is not a rare cause of Aeromonas bacteremia in Hiroshima, Japan[J]. Journal of Infection and Chemotherapy:Official Journal of the Japan Society of Chemotherapy,2020, 26(2):316-320.
[2] CHEN P L, WU C J, CHEN C S, et al. A comparative study of clinical Aeromonas dhakensis and Aeromonas hydrophila isolates in southern Taiwan:A. dhakensis is more predominant and virulent[J]. Clinical Microbiology and Infection, 2014, 20(7):O428-O434.
[3] HEERMANN R, JUNG K. The complexity of the ‘simple’two-component system KdpD/KdpE in Escherichia coli[J].FEMS Microbiology Letters, 2010, 304(2):97-106.
[4] FREEMAN Z N, DORUS S, WATERFIELD N R. The Kdp D/kdp E two-component system:integrating K+homeostasis and virulence[J]. PLoS Pathogens, 2013, 9(3):e1003201.
[5] 丁海峰,李小申,黄慧,等.基因敲除技术研究进展[J].黑龙江畜牧兽医, 2018(5):50-54.
[6]

WANG C, SUN H. Progress in gene knockout mice[J].Sheng Wu Gong Cheng Xue Bao, 2019, 35(5):784-794.
[7]

LIU Z, LIU P, LIU S, et al. Small protein B upregulates sensor kinase bvg S expression in Aeromonas veronii[J].Frontiers in Microbiology, 2015, 16:6:579.
[8]

SONG T, TOMA C, NAKASONE N, et al. Aerolysin is activated by metalloprotease in Aeromonas veronii biovar sobria[J]. Journal of Medical Microbiology, 2004, 53(6):477-482.
[9]

SILVER A C, RABINOWITZ N M, KUF FER S, et al.Identification of Aeromonas veronii genes required for colonization of the medicinal leech, Hirudo verbana[J]. Journal of Bacteriology, 2007, 189(19):6763-6772.
[10]

WEI Z, FU Q, CHEN Y, et al. The capsule of Streptococcus equi ssp. zooepidemicus is a target for attenuation in vaccine development[J]. Vaccine, 2012, 30(31):4670-4675.
[11]

QUÉNÉE L, LAMOTTE D, POLACK B. Combined sacBbased negative selection and cre-lox antibiotic marker recycling for efficient gene deletion in Pseudomonas aeruginosa[J]. BioTechniques, 2005, 38(1):63-67.
[12]

AMBROSIS N, FERNÁNDEZ J, SISTI F. Counterselection method for markerless allelic exchange in Bordetella bronchiseptica based on sac B gene from Bacillus subtilis[J]. Current Protocols in Microbiology, 2020, 59(1):e125.
[13]

TAKAMATSU D, OSAKI M, SEKIZAKI T. Thermosensitive suicide vectors for gene replacement in Streptococcus suis[J]. Plasmid, 2001, 46(2):140-148.
[14] 常雅洁,丁雪燕,王思权,等.常见细菌基因敲除技术的研究进展[J].中国预防兽医学报, 2021, 43(10):1118-1123.
[15] 李颖平,张映,邵国青.一种新的基因敲除术-RNAi[J].生物技术通报, 2006(3):42-45.
[16] 李今煜,陈健铭,彭振坤.几种常用的基因敲除技术[J].武夷科学, 2007, 23(0):187-190.
[17]

MILLER J C, HOLMES M C, WANG J, et al. An improved zinc-finger nuclease architecture for highly specific genome editing[J]. Nature Biotechnology, 2007,25(7):778-785.
[18]

GAJ T, GERSBACH C A, BARBAS III C F. ZFN,TALEN, and CRISPR/Cas-based methods for genome engineering[J]. Trends in Biotechnology, 2013, 31(7):397-405.
[19]

JOUNG J, KONERMANN S, GOOTENBERG J S, et al.Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening[J]. Nature Protocols, 2017, 12(4):828-863.
[20]

DALVIE N C, LORGEREE T, BIEDERMANN A M, et al. Simplified gene knockout by CRISPR-Cas9-induced homologous recombination[J]. ACS Synthetic Biology,2022, 11(1):497-501.
[21]

ALI M K, LI X, TANG Q, et al. Regulation of inducible potassium transporter KdpFABC by the KdpD/KdpE two-component system in Mycobacterium smegmatis[J].Frontiers in Microbiology, 2017, 8:570.
[22] 余华,熊浚智,何晓梅,等.采用Red重组系统敲除铜绿假单胞菌弹性蛋白酶基因[J].中国人兽共患病学报, 2013, 29(2):129-132.
[23]

LIU R, ZHANG P, SU Y, et al. A novel suicide shuttle plasmid for Streptococcus suis serotype 2 and Streptococcus equi ssp. zooepidemicus gene mutation[J]. Scientific Reports, 2016, 6:27133.
[24] 吴菁,刘秀敏,张维,等.枯草芽孢杆菌sac B基因的功能验证及应用[J].核农学报, 2008, 22(5):590-594.
[25]

SUN X, YANG D, WANG Y, et al. Development of a markerless gene deletion system for Streptococcus zooepidemicus:functional characterization of hyaluronan synthase gene[J]. Applied Microbiology and Biotechnology,2013, 97(19):8629-8636.
[26]

HOOVEN T A, BONAKDAR M, CHAMBY A B, et al. A counterselectable sucrose sensitivity marker permits efficient and flexible mutagenesis in Streptococcus agalactiae[J]. Applied and Environmental Microbiology, 2019,85(7):e03009-e03018.
[27]

SCHÄFER A, TAUCH A, JÄGER W, et al. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19:selection of defined deletions in the chromosome of Corynebacterium glutamicum[J]. Gene, 1994, 145(1):69-73.